Cry蛋白对多种马铃薯害虫的杀虫效果鉴定  

Identification of the Insecticidal Effect of Cry Protein on a Variety of Potato Pests

作　　者：周德山 杨逗逗 刘娟 林卫潮 程洁 李素华 ZHOU Deshan;YANG Doudou;LIU Juan;LIN Weichao;CHENG Jie;LI Suhua(College of Agronomy,Qingdao Agricultural University,Qingdao 266109,China;Shenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture/Key Laboratory of Synthetic Biology,Ministry of Agriculture and Rural Affairs/Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518000,China)

机构地区：[1]青岛农业大学农学院,山东青岛266109 [2]岭南现代农业科学与技术广东省实验室深圳分中心/农业农村部合成生物学重点实验室/中国农业科学院(深圳)农业基因组研究所,广东深圳518000

出　　处：《广东农业科学》2024年第10期62-69,共8页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(32302375);广东省重大人才工程引进项目(2021QN02N756)。

摘　　要：【目的】研究Cry蛋白对马铃薯甲虫(Leptinotarsa decemlineata)、草地贪夜蛾(Spodoptera frugiperda)、茄二十八星瓢虫(Henosepilachna vigintioctopunctata)3种马铃薯害虫的杀虫效果,为创制Cry转基因马铃薯抗虫植株提供基础。【方法】以‘希森6号’‘荷兰15’马铃薯为供试材料,上述3种害虫为供试昆虫,采用饲料添加法和叶片涂抹法施加Cry蛋白(Cry1B、Cry1Ac、Cry3A、Cry3B),设置无菌水(CK)和不同浓度的Cry蛋白处理组。通过比较各处理组间的幼虫死亡率和虫体质量来确定Cry蛋白对3种害虫的杀虫效果。【结果】Cry3A蛋白在10、20、100μg/g处理浓度下均能显著抑制2龄马铃薯甲虫幼虫虫体质量并提高其死亡率,而同浓度Cry1B蛋白处理与对照CK相比对2龄马铃薯甲虫幼虫的虫体质量无明显影响,表明Cry1B蛋白对该幼虫无杀虫效果。使用100μg/gCry1Ac和Cry1B蛋白分别饲喂草地贪夜蛾发现,Cry1Ac蛋白能够显著抑制草地贪夜蛾幼虫的生长,而Cry1B处理与CK相比并未显著影响幼虫虫体质量,表明Cry1B蛋白对草地贪夜蛾无杀虫效果。使用100μg/g Cry1B蛋白处理2龄茄二十八星瓢虫幼虫,其在抑制幼虫虫体质量和提高其死亡率方面有显著效果,其中第2天和第3天死亡率分别为50%和100%,表明Cry1B蛋白对茄二十八星瓢虫有良好的杀虫效果。【结论】Cry1Ac蛋白对草地贪夜蛾有杀虫效果,Cry3A蛋白对马铃薯甲虫有杀虫效果。而Cry1B蛋白对茄二十八星瓢虫有杀虫效果,但对马铃薯甲虫、草地贪夜蛾无杀虫效果。【Objective】The study was conducted to explore the insecticidal effects of Cry protein on Leptinotarsa decemlineata,Spodoptera frugiperda,and Henosepilachna vigintioctopunctata,aiming to provide a foundation for the development of Cry transgenic potato plants with insect resistance.【Method】‘Xisen 6’and‘Favorita 15’potatoes were taken as the test materials and the aforementioned three pest insects as the test subjects.The Cry proteins(Cry1B,Cry1Ac,Cry3A,Cry3B)was applied by using both feed addition and leaf coating methods,and sterile water(CK)and different concentrations of Cry protein treatment groups were set up.The insecticidal effects of the Cry protein on the three insects were identified by comparing the larval mortality rate and biomass among different treatments.【Result】Cry3A protein at concentrations of 10,20,and 100μg/g showed significant effects in inhibiting the biomass and increasing the mortality rate of 2nd instar L.decemlineata.In contrast,under the same concentration,there was no significant difference in the biomass of 2nd instar L.decemlineata treated with Cry1B protein compared with the CK,indicating that Cry1B protein had no insecticidal effect on these larvae.Under the treatments with 100μg/g of Cry1Ac and Cry1B proteins to feed S.frugiperda respectively,the results showed that Cry1Ac protein significantly inhibited the growth of S.frugiperda larvae,while there was no significant difference in larval biomass between the treatments with Cry1B and CK,indicating that Cry1B protein had no insecticidal effect on S.frugiperda.Treatment of 2nd instar H.vigintioctopunctata with 100μg/g of Cry1B protein exhibited significant effects in inhibiting larval biomass and increasing mortality rate,with the mortality rates on the second day and third day being 50%and 100%,respectively.The results indicated that Cry1B protein had strong insecticidal effect on H.vigintioctopunctata.【Conclusion】Cry1Ac protein has a insecticidal effect on S.frugiperda,Cry3A protein exhibits insecticidal

关 键 词：Cry1B蛋白 马铃薯甲虫 草地贪夜蛾 茄二十八星瓢虫 杀虫效果 生物测定 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]