营养盐对波吉卵囊藻生长性能的影响  

Effects of Nutrient Salts on Growth Performance of Oocystis borgii

作　　者：张彬 林原有 原宝龙 朱昔恩 陈田聪 赵永贞 申乃坤 ZHANG Bin;LIN Yuanyou;YUAN Baolong;ZHU Xien;CHEN Tiancong;ZHAO Yongzhen;SHEN Naikun(Guangxi Academy of Fisheries Science/Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture,Nanning 530021,China;School of Marine Sciences and Biotechnology,Guangxi Minzu University/Guangxi Key Laboratory of Polysaccharide Materials and Modification,Nanning 530006,China)

机构地区：[1]广西水产科学研究院/广西水产遗传育种与健康养殖重点实验室,广西南宁530021 [2]广西民族大学海洋与生物技术学院/广西多糖材料与改性重点实验室,广西南宁530006

出　　处：《广东农业科学》2024年第10期88-96,共9页Guangdong Agricultural Sciences

基　　金：广西科技重大专项(桂科AA23062047);国家现代农业产业技术体系专项(CARS-48-53);广西水产科学研究院结余经费科技项目(GXIFJY-202310)。

摘　　要：【目的】研究氮、磷、碳和铁营养盐对波吉卵囊藻(Oocystis borgei)生长的影响,筛选出最佳营养盐配方,为其高效扩培提供参考。【方法】先利用单因素试验分析不同氮、磷、碳和铁源对波吉卵囊藻生长的影响,获得各自最佳营养盐及最适浓度,再利用正交试验对波吉卵囊藻生长影响较大因素进行优化,得出最佳营养盐配方。【结果】单因素试验结果表明,波吉卵囊藻的最佳氮、磷、碳和铁源及浓度分别是:CO(NH_(2))_(2)160 mg/L、C_(3)H_(7)Na_(2)O_(6)P 8 mg/L、NaHCO_(3)1 g/L、FeCl_(3)0.2 mg/L;正交试验结果表明,各营养盐对波吉卵囊藻生长影响大小为:磷源>氮源>碳源,其中磷源对波吉卵囊藻生长影响最为显著(P<0.05),最佳营养盐配方为:CO(NH_(2))_(2)120 mg/L、C_(3)H_(7)Na_(2)O_(6)P 12 mg/L、NaHCO_(3)2.5 g/L,最优条件下藻细胞密度可达22.07×10^(6)cell/mL,比优化前(1.74×10^(6)cell/mL)提高12倍以上。【结论】确定了波吉卵囊藻最适氮、磷、碳和铁源及其浓度,且通过优化条件得出最佳营养盐配方,显著提高藻细胞密度。【Objective】The effects of nitrogen,phosphorus,carbon and iron nutrients on the growth of Oocystis borgei were explored,and the optimal nutrient formula was selected to provide references for its efficient and large-scale cultivation.【Method】Single factor test was used to analyze the effects of different nitrogen,phosphorus,carbon and iron nutrients on the growth of O.borgei,to obtain the best nutrients and their respective concentrations.Orthogonal tests were conducted to optimize the factors that had a great impact on the the growth of O.borgei.Finally,the optimal nutrient formula was obtained.【Result】The results of the single factor test showed that the optimal sources and concentrations of nitrogen,phosphorus,carbon and iron sources for O.borgei were CO(NH_(2))_(2)160 mg/L、C_(3)H_(7)Na_(2)O_(6)P 8 mg/L、NaHCO_(3)1 g/L and FeCl_(3)0.2 mg/L,respectively.The results of the orthogonal tests showed that the effect of each nutrient on the growth of O.borgei ranked as:phosphorus source>nitrogen source>carbon source,and phosphorus source had the most significant effect on the growth of O.borgei(P<0.05).The optimal formula was:CO(NH_(2))_(2)120 mg/L、C_(3)H_(7)Na_(2)O_(6)P 12 mg/LandNaHCO_(3)2.5 g/L.Under the optimal condition,the algal cell density reached 22.07×10^(6) cells/mL,which was 12 times higher than that before optimization(1.74×10_(6) cells/mL).【Conclusion】The optimal nitrogen,phosphorus,carbon,and iron sources and concentrations for O.borgei were determined and the optimal nutrient formula was obtained by optimizing the conditions,which significantly increased the algal cell density.

关 键 词：波吉卵囊藻 氮源 磷源 碳源 铁源 营养盐 

分 类 号：S968.4[农业科学—水产养殖]