穴位埋线联合温肾补骨方治疗绝经后乳腺癌芳香化酶抑制剂相关骨关节症状的临床研究  

Mechanism of Garcinia Yunnanensis Extract effecting on invasion and metastasis of colorectal cancer cell

作　　者：邹璇 施茵[2] 吴焕淦[3] 陈子怡 张惜音 杨茗橘 王儒蒙 张馨月[1] 李琼[1] 陈佳莹 柳光宇[4] 汤立晨[4] 陈建刚 季亚婕[1] 薛晓红[1] ZOU Xuan;SHI Yin;WU Huangan;CHEN Ziyi;ZHANG Xiyin;YANG Mingju;WANG Rumeng;ZHANG Xinyue;LI Qiong;CHEN Jiaying;LIU Guangyu;TANG Lichen;CHEN Jiangang;JI Yajie;XUE Xiaohong(Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China;Shanghai Seventh People's Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200120,China;Shanghai Research Institute of Acupuncture and Meridian,Shanghai 200030,China;Fudan University Shanghai Cancer Center,Shanghai 200032,China;School of Communication&Electronic Engineering,East China Normal University,Shanghai 200241,China)

机构地区：[1]上海中医药大学附属岳阳中西医结合医院,上海200437 [2]上海中医药大学附属第七人民医院,上海200120 [3]上海市针灸经络研究所,上海200030 [4]复旦大学附属肿瘤医院,上海200032 [5]华东师范大学通信与电子工程学院,上海200241

出　　处：《上海中医药大学学报》2024年第6期32-38,共7页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：国家重点研发计划项目(2023YFF1205004);国家自然科学基金资助项目(82151318,82004446);上海市科委“科技创新行动计划”医学创新研究专项项目(22Y11923000,23Y11920900D);上海市中医药学会“凯宝”青年医师海派中医传承研究项目(2023-HPZY-04);上海市针灸临床医学研究中心项目(20MC1920500);国家中医药领军人才支持计划-岐黄学者项目;中医专科(中医乳腺专科)建设项目[YW(2023-2024)-01-06];薛晓红名中医工作室项目。

摘　　要：目的:观察穴位埋线联合温肾补骨方治疗绝经后乳腺癌芳香化酶抑制剂相关骨关节症状(AIMSS)的临床疗效。方法:采用随机对照试验设计,将128例绝经后乳腺癌AIMSS患者随机分为线药结合组和单纯中药组,每组各64例。两组患者均口服温肾补骨方汤剂,线药结合组患者在此基础上加用穴位埋线,两组治疗周期均为12周,疗程结束后随访12周。于治疗前及治疗第6、12、18、24周,评估两组患者的简明疼痛量表-简表(BPI-SF)和乳腺癌患者生命质量评定量表(FACT-B)评分;于治疗前及治疗第12、24周,评估两组患者的Morisky服药依从性量表(MMAS-8)评分。结果:研究过程中,线药结合组剔除3例、脱落4例,单纯中药组剔除1例、脱落6例,最终线药结合组61例、单纯中药组63例纳入基于修正的意向性治疗人群(mITT)分析。(1)治疗后,两组患者的简明疼痛量表-最剧烈疼痛(BPI-WP)评分、平均疼痛评分、疼痛程度评分及疼痛干扰评分较治疗前均明显降低(P<0.01),且与单纯中药组相比,治疗第12、18、24周线药结合组患者的BPI-WP评分明显降低(P<0.05),治疗第12、18周线药结合组患者的平均疼痛评分、疼痛程度评分明显降低(P<0.05),治疗第12周线药结合组患者的疼痛干扰评分明显降低(P<0.05)。(2)治疗第6、12、18、24周,两组患者的FACT-B评分较治疗前均明显升高(P<0.01),且线药结合组患者的评分高于单纯中药组(P<0.05)。(3)治疗第12、24周,两组患者的MMAS-8评分较治疗前均明显升高(P<0.01),但两组评分组间比较差异无统计学意义(P>0.05)。结论:与单纯中药治疗相比,穴位埋线联合温肾补骨方的中医综合疗法能更有效地缓解绝经后乳腺癌AIMSS患者的关节疼痛,提高患者的生存质量,且疗效具有持续性,值得临床推广。Objective:To explore the inhibitory effects and its potential mechanisms of YTE-17,a standard ethanolic extract of Garcinia Yunnanensis,on the metastasis of colorectal cancer.Methods:Clone formation assay was used to observe the growth and proliferation of colorectal cancer cells HCT116 and LoVo treated with YTE-17.Scratch test and Transwell assay were performed to detect the effect of YTE-17 on the invasion and migration ability of colorectal cancer.Western blot analysis was conducted to detect the regulatory effect of YTE-17 on the expressions of MMP-2,Survivin,Cyclin D1,E-cadherin,and Vimentin.Results:YTE-17 significantly inhibited the growth and proliferation of colorectal cancer cells HCT116 and LoVo.Compared with the control group,YTE-17 suppressed the invasion and migration abilities of HCT116 and LoVo cells(P<0.01)in a dose-dependent manner.Western blot results revealed that YTE-17reduced the expressions of Survivin,Cyclin D1 and E-cadherin proteins in HCT116 cells in a dose-dependent manner(P<0.01),and reduced the expressions of Survivin,Cyclin D1,MMP-2 and Vimentin proteins in LoVo cells in a dose-dependent manner(P<0.01).Conclusion:YTE-17 can inhibit the invasion and metastasis of colorectal cancer by reducing the expressions of Survivin,Cyclin D1,MMP-2,E-cadherin and Vimentin proteins.

关 键 词：芳香化酶抑制剂相关骨关节症状 绝经后乳腺癌 穴位埋线 温肾补骨方 中医药疗法 

分 类 号：R273[医药卫生—中西医结合]