DNA甲基化研究的分析前影响因素  

Pre-analytical influencing factors in DNA methylation study

作　　者：胡松年 丁晖[1,2,3] 赵立芳 蔡彦宁 HU Song-Nian;DING Hui;ZHAO Li-Fang;Cai Yan-Ning(Neurobiology Laboratory,Xuanwu Hospital,Capital Medical University,Beijing 100053,China;Key Laboratory of Neurodegenerative Diseases,Ministry of Education,Beijing 100053,China;National Clinical Research Center for Geriatric Diseases,Beijing 100053,China;Clinical Sample Center,Xuanwu Hospital,Capital Medical University,Beijing 100053,China)

机构地区：[1]首都医科大学宣武医院神经生物学研究室,北京100053 [2]教育部神经变性病重点实验室,北京100053 [3]国家老年疾病临床医学研究中心,北京100053 [4]首都医科大学宣武医院临床样本中心,北京100053

出　　处：《生命科学》2024年第11期1330-1338,共9页Chinese Bulletin of Life Sciences

基　　金：国家重点研发计划(2021YFC2501205)。

摘　　要：DNA甲基化是一种导致基因表达沉默的动态表观遗传修饰,在临床疾病生物标志物研究中受到了广泛关注。然而,由于缺乏标准化的分析前处理方式,当前DNA甲基化分析结果的准确性和可靠性无法保证。本文旨在探讨不同分析前因素对DNA甲基化分析结果的影响,并对部分因素给出适当的处理建议,以期为DNA甲基化相关临床研究提供新见解。DNA methylation is a dynamic epigenetic modification that leads to silencing of gene expression and has received extensive attention in clinical biomarker study.However,the accuracy and reliability of current DNA methylation analysis are uncertain due to lacking of standardized pre-analysis procedures.The purpose of this paper is to explore the influence of different factors on DNA methylation analysis and to give appropriate treatment suggestions for some factors to provide new insights for clinical research on DNA methylation.

关 键 词：DNA 甲基化 分析前影响因素 

分 类 号：Q34[生物学—遗传学]