PAI-1和TLR4在烧伤后增生性瘢痕组织中的表达水平及意义  

Expression levels and significance of PAI-1 and TLR4 in hypertrophic scar tissue after burn

作　　者：孙佳琳 郑伟才[1] 樊磊 SUN Jialin;ZHENG Weicai;FAN Lei(Department of Burn Plastic Surgery,First Affiliated Hospital of Henan University of Science and Technology,Luoyang,He'nan 471000,China)

机构地区：[1]河南科技大学第一附属医院烧伤整形外科,河南洛阳471000

出　　处：《安徽医药》2025年第1期79-83,I0005,共6页Anhui Medical and Pharmaceutical Journal

基　　金：洛阳市科技计划项目(2101039A)。

摘　　要：目的探究纤溶酶原激活物抑制剂-1(PAI-1)和Toll样受体4(TLR4)在烧伤后增生性瘢痕(HS)组织中的表达水平及临床意义。方法选取河南科技大学第一附属医院2020年7月至2022年7月收治的94例HS病人为研究对象,术中取病人HS组织为HS组,并选取HS病人正常皮肤组织为对照组。实时荧光定量逆转录聚合酶链反应(qRT-PCR)法对组织中PAI-1、TLR4 mRNA表达水平进行测定;免疫组化法对组织中PAI-1、TLR4阳性表达情况进行测定;采用蛋白质印迹法测定PAI-1、TLR4的蛋白表达水平;使用温哥华瘢痕量表(VSS)对瘢痕组织严重情况进行评价,使用跨皮水分丢失(TEWL)对HS病人的表皮屏障功能进行评估;Pearson相关性分析病人HS组织中PAI-1、TLR4表达水平与VSS及TEWL净丧失量之间的关系。结果HS组PAI-1表达水平1.54±0.32、TLR4 mRNA表达水平1.61±0.38高于对照组1.11±0.24、1.06±0.21,HS组PAI-1、TLR4阳性表达率及蛋白表达水平也显著高于对照组(P<0.05);病人HS组织VSS评分为(12.01±3.48)分,HS组织TEWL值显著高于对照组(P<0.05),TEWL净丧失量为(7.87±2.37)g·m^(−2)·h^(−1);Pearson相关性分析结果表明,HS组织中PAI-1、TLR4表达水平分别与VSS及TEWL净丧失量呈显著正相关(P<0.05)。结论烧伤后病人HS组织中PAI-1、TLR4 mRNA表达水平、阳性表达率及蛋白表达水平均显著升高,且PAI-1、TLR4与HS组织严重程度,表皮屏障功能障碍有关,因此PAI-1、TLR4有望成为临床治疗HS的新靶点。Objective To investigate the expression levels and clinical significance of plasminogen activator inhibitor-1(PAI-1)and Toll-like receptor 4(TLR4)in hypertrophic scar(HS)tissue after burn.Methods Ninety-four patients with HS who were admitted to the First Affiliated Hospital of He´nan University of Science and Technology from July 2020 to July 2022 were selected as the study subjects.The HS tissues of patients were selected as the HS group during the operation,and the normal skin tissues of HS patients were selected as the control group.The expression levels of PAI-1 and TLR4 mRNA in tissues were measured by fluorescence quantitative PCR(qRT-PCR);the positive expression of PAI-1 and TLR4 in tissues was determined by immunohistochemistry;Western blotting was applied to determine the protein expression levels of PAI-1 and TLR4;the severity of scar tissue was evaluated using the Vancouver Scar Scale(VSS),and the epidermal barrier function of HS patients was evaluated using transcutaneous water loss(TEWL);Pearson correlation was applied to analyze the relationship between the expression levels of PAI-1 and TLR4 in HS tissue and the VSS and net loss of TEWL.Results PAI-1(1.54±0.32)and TLR4(1.61±0.38)mRNA expression levels in HS group were higher than those in control group(1.11±0.24)and(1.06±0.21),and the positive expression rates and protein expression level of PAI-1 and TLR4 in HS group were also obviously higher than those in control group(P<0.05);the VSS score of HS tissue was(12.01±3.48),and the TEWL value of HS tissue was obviously higher than that of the control group(P<0.05),the net loss of TEWL was(7.87±2.37)g·m^(−2)·h^(−1);Pearson correlation analysis showed that the expression levels of PAI-1 and TLR4 in HS tissue were obviously positively correlated with the VSS and net loss of TEWL,respectively(P<0.05).Conclusion The mRNA expression levels,positive expression rates and protein expression of PAI-1 and TLR4 in HS tissue of burn patients are obviously increased,PAI-1 and TLR4 are related to the

关 键 词：纤溶酶原激活物抑制物 TOLL样受体4 烧伤 增生性瘢痕 临床意义 

分 类 号：R622[医药卫生—整形外科]