肾病综合征病人血清lncRNA ANRIL和BDNF水平变化与血栓栓塞的相关性分析  

Correlation analysis between changes of serum lncRNA ANRIL and BDNF levels and thromboembolism in patients with nephrotic syndrome

作　　者：顾玉[1] 梁晓艳[2] 马胜辉[1] 佟娜 阎泽君[1] GU Yu;LIANG Xiaoyan;MA Shenghui;TONG Na;YAN Zejun(Laboratory Department of Chengde Central Hospital,Chengde,Hebei 067000,China;Basic Department,Chengde Nursing Vocational College,Chengde,Hebei 067000,China)

机构地区：[1]承德市中心医院检验科,河北承德067000 [2]承德护理职业学院基础部,河北承德067000

出　　处：《安徽医药》2025年第1期141-146,共6页Anhui Medical and Pharmaceutical Journal

基　　金：承德市科学技术研究课题计划项目(202002A017)。

摘　　要：目的探究肾病综合征(NS)病人血清长链非编码RNA(lncRNA)细胞周期激酶抑制因子4基因座中反义非编码RNA(ANRIL)和脑源性神经营养因子(BDNF)水平变化与血栓栓塞(TE)的相关性。方法选取2019年3月至2021年12月在承德市中心医院住院治疗的220例NS病人为研究对象,根据随访结果将其分为NS组118例和TE组102例,另外选取同期体检健康者100例为对照组。采用实时荧光定量PCR(qRT-PCR)测定研究对象血清lncRNA ANRIL水平;采用酶联免疫吸附测定(ELISA)检测研究对象血清BDNF水平;使用全自动生化分析仪检测相关生化指标;采用Pearson相关性分析血清lncRNA ANRIL、BDNF水平与生化指标相关性;采用多因素logistic回归分析NS病人发生TE的影响因素;采用受试者操作特征曲线(ROC曲线)分析血清lncRNA ANRIL、BDNF在预测NS病人发生TE中的价值。结果与对照组相比较,NS组、TE组血清lncRNA ANRIL水平显著升高(2.54±0.43比5.82±1.34、8.35±2.17),BDNF水平显著降低[(32.77±8.25)μg/L比(24.49±4.58)μg/L、(18.63±3.62)μg/L](P<0.05);与NS组相比较,TE组血清lncRNA ANRIL水平显著升高,BDNF水平显著降低(P<0.05);对照组、NS组、TE组三组的尿酸、血肌酐、乳酸脱氢酶、血尿素氮、尿蛋白水平呈逐渐升高趋势,血清白蛋白、血红蛋白水平呈逐渐降低趋势(P<0.05)。Pearson相关性分析显示,NS合并TE病人血清lncRNA ANRIL水平与尿酸、血肌酐、乳酸脱氢酶、血尿素氮、尿蛋白水平呈正相关,与血清白蛋白、血红蛋白水平呈负相关(P<0.05);NS合并TE病人血清BDNF水平与尿酸、血肌酐、乳酸脱氢酶、血尿素氮、尿蛋白水平呈负相关,与血清白蛋白、血红蛋白水平呈正相关(P<0.05)。多因素logistic回归分析显示,lncRNA ANRIL、尿蛋白和BDNF是NS病人发生TE的影响因素(P<0.05);ROC曲线结果显示,血清lncRNA ANRIL、BDNF预测NS病人发生TE的曲线下面积(AUC)分别为0.84、0.85,两者联合预测的AUC为0.92Objective To investigate the correlation between the changes of long non-coding RNA(lncRNA)antisense non-coding RNA in the INK4 locus(ANRIL)and brain-derived neurotrophic factor(BDNF)levels and thromboembolism(TE)in patients with nephrotic syndrome(NS).Methods Two hundred and twenty NS patients who were hospitalized in Chengde Central Hospital from March 2019 to October 2021 were selected as the study subjects,including 102 NS patients with TE as the TE group,118 NS patients without TE as the NS group,and 100 healthy persons in the same period were regarded as the control group.Real-time fluorescence quantitative PCR(qRT-PCR)was applied to measure the serum lncRNA ANRIL level of the subjects;the serum BDNF level of the subjects was detected by enzyme-linked immunosorbent assay(ELISA);automatic biochemical analyzer was applied to detect relevant biochemical indicators;Pearson correlation was applied to analyze the correlation between serum lncRNA ANRIL,BDNF levels and biochemical indicators;multivariate Logistic regression was applied to analyze the influencing factors of TE in NS patients;the predictive value of serum lncRNA ANRIL and BDNF on the occurrence of TE in NS patients was analyzed by using the Receiver operating Characteristic curve(ROC curve).Results Compared with the control group,the serum lncRNA ANRIL level in NS group and TE group was significantly increased(2.54±0.43 vs.5.82±1.34,8.35±2.17).The level of BDNF was significantly decreased[(32.77±8.25)μg/L vs.(24.49±4.58)μg/L,(18.63±3.62)μg/L](P<0.05).Compared with NS group,the serum lncRNA ANRIL level in TE group was significantly increased,and the serum BDNF level was significantly decreased(P<0.05).The levels of uric acid,serum creatinine,lactate dehydrogenase,blood urea nitrogen,and urine protein in the control group,NS group and TE group were gradually increased,while the levels of serum albumin,and hemoglobin were gradually decreased(P<0.05).Pearson correlation analysis showed that the level of serum lncRNA ANRIL was positively correlat

关 键 词：肾病综合征 非编码RNA ANRIL 脑源性神经营养因子 血栓栓塞 预测价值 

分 类 号：R692[医药卫生—泌尿科学]