姜黄素通过抑制大鼠冠状动脉微栓塞后Wnt5a/β-Catennin通路减轻心肌炎症反应  

Curcumin alleviates myocardial inflammatory responses by inhibiting Wnt5a/β-Catennin pathway after coronary microembolization in rats

作　　者：易泽强 李涛[1] 农家聪 涂光鹏 李浪[1,2,3] YI Zeqiang;LI Tao;NONG Jiacong;TU Guangpeng;LI Lang(The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China;Guangxi Key Laboratory of Precision Medicine in Cardiocerebrovascular Diseases Control and Prevention,Guangxi Medical University,Nanning 530021,China;Guangxi Clinical Medical Research Center of Cardiovascular and Cerebrovascular Diseases,Nanning 530021,China)

机构地区：[1]广西医科大学第一附属医院,南宁530021 [2]广西医科大学广西心脑血管疾病防治精准医学重点实验室,南宁530021 [3]广西心脑血管疾病临床医学研究中心,南宁530021

出　　处：《广西医科大学学报》2024年第12期1581-1587,共7页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.81770346)。

摘　　要：目的:探索姜黄素对大鼠冠状动脉微栓塞(CME)后心肌炎症反应的潜在机制。方法:将32只SD大鼠随机分为假手术组、CME组(模型组)、CME+姜黄素组(姜黄素组)、CME+姜黄素+Foxy-5(激动剂组),每组8只。通过夹闭大鼠升主动脉并在左心室注射惰性聚苯乙烯微球构建大鼠CME模型。采用酶联免疫吸附试验(ELISA)检测血清中心肌肌钙蛋白Ⅰ(cTnⅠ)水平,苏木精-伊红(HE)染色观察大鼠心肌病理改变,实时荧光定量PCR(RT-qPCR)和蛋白免疫印迹(western blotting)法检测白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、无翅型小鼠乳腺肿瘤病毒整合位点家族成员5α(Wnt5α)和β-连环蛋白(β-Catennin)在心肌中的转录及蛋白表达水平。结果:与假手术组相比,模型组大鼠心功能显著下降,TNF-α、IL-1β、Wnt5a的转录及蛋白表达量均显著升高,而β-Catennin表达下调(均P<0.05)。与模型组相比,姜黄素组大鼠心功能升高,TNF-α、IL-1β、Wnt5a的转录及蛋白表达量显著下降,β-Catennin表达上调(均P<0.05)。激动剂Foxy-5显著逆转了姜黄素对上述指标的作用(P<0.05)。结论:姜黄素可通过抑制大鼠CME后Wnt5a/β-Catennin通路来减轻心肌炎症反应。Objective:To explore the potential mechanisms of curcumin on myocardial inflammatory responses following coronary microembolization(CME)in rats.Methods:Thirty-two SD rats were randomly divided into a sham group,a CME group(model group),a CME+curcumin group(curcumin group),and a CME+curcumin+Foxy-5 group(agonist group),with 8 rats in each group.The CME model in rats was established by clamping the ascending aorta and injecting inert polystyrene microspheres into the left ventricle.Real-time fluorescent quantita‐tive PCR(RT-qPCR)and western blotting(WB)were employed to detect the transcription and translation levels of interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),Wnt family member 5a(Wnt5a),andβ-catenin in the myocardium.Results:Compared with the sham group,rats in the model group exhibited significantly decreased cardiac function,along with significantly elevated transcription and translation levels of TNF-α,IL-1β,and Wnt5a,whileβ-catenin expression levels showed a decrease(all P<0.05).Compared with the model group,rats in the curcumin group showed an increasing trend in cardiac function,with significantly decreased transcription and translation levels of TNF-α,IL-1β,and Wnt5a,and an increase inβ-catenin expression levels(all P<0.05).The agonist Foxy-5 significantly reversed the effects of curcumin on the above indexes(P<0.05).Conclusion:Curcumin can alleviate myocardial inflammatory responses by inhibiting the Wnt5a/β-catenin pathway following CME in rats.

关 键 词：姜黄素 冠状动脉微栓塞 炎症反应 

分 类 号：R541.4[医药卫生—心血管疾病]