基于LC-MS和GC-MS代谢组学分析头花蓼不同入药部位黄酮类成分  

Analysis of flavonoids in the different parts of Polygonum capitatum via LC-MS and GC-MS metabolomics technology

作　　者：彭启伦 杨杰 郭步伐 叶茂 易伟 PENG Qi-lun;YANG Jie;GUO Bu-fa;YE Mao;YI Wei(Bijie Medical College,Bijie 551700,China;Department of Pharmacy,Guangzhou Medical University,Guangzhou 511495,China)

机构地区：[1]毕节医学高等专科学校,毕节551700 [2]广州医科大学药学院,广州511495

出　　处：《天然产物研究与开发》2024年第12期2031-2041,共11页Natural Product Research and Development

基　　金：贵州省基础研究计划(黔科合基础-ZK[2023]一般003);毕节市科技创新平台及人才团队(毕科合[2023]66号)。

摘　　要：为了探究头花蓼不同入药部位黄酮类化合物分布的丰度,挖掘其潜在的药用价值。采用LC-MS和GC-MS代谢组学技术对头花蓼茎、花和叶进行黄酮类化合物代谢组学比较研究。结果表明:头花蓼花中黄酮类化合物分布的数量(425个)>叶(376个)>茎(354个);以差异倍数(fold change)≥2或≤0.5,P<0.05为依据在头花蓼茎与花、茎与叶及叶与花对比中分别筛选到333个、289个和366个黄酮类差异代谢物。头花蓼三组样本对比(茎与花、茎与叶、叶与花)的差异代谢物显著富集于黄酮生物合成途径。3′,4′,5′,5,7-五羟黄酮、表儿茶素和表没食子儿茶素在花中的相对含量显著高于茎或叶,并且是重要的药理活性物质。本研究全面分析和鉴定了头花蓼不同入药部位的黄酮类化合物,证实了黄酮类化合物主要分布在花,为后续深入挖掘和利用其黄酮类化合物的药用价值提供理论基础。The aim of this study is to explore the differential distribution of flavonoids in the different parts of Polygonum capitatum and dig its potential medicinal value.Therefore,LC-MS and GC-MS metabolomics technology was used in comparatively researching the flavonoids in the stems,flowers and leaves of P.capitatum.It was established that the number of flavonoids in the P.capitatum aboveground part was ranked in descending order as follows:flower>leaf>stem.Additionally,based on the screening criteria(fold change≥2 or≤0.5,P<0.05)of differentially expressed metabolites(DEMs),we obtained 333,289 and 366 DEMs in three groups of PC sample comparison(stems and flowers,stems and leaves,leaves and flowers),respectively.Subsequently,the DEMs in different medicinal parts of P.capitatum were significantly enriched in flavonoid biosynthesis pathway.In these DEMs,the relative concentration of these flavonoids(3′,4′,5′,5,7′-pentahydroxyflavone,epicatechin and epigallocatechin)in the flowers of P.capitatum was greater than that in the stems or leaves.Meanwhile,3′,4′,5′,5,7′-pentahydroxyflavone,epicatechin and epigallocatechin are important pharmacological active substance.The research comprehensively identified the flavonoids in the different medicinal parts of P.capitatum,and affirmed that the flavonoids were majorly distributed in the flower.These results provided the theoretical basis for deeply digging and utilizing the medicinal value of its flavonoids.

关 键 词：头花蓼 不同入药部位 代谢组学 黄酮 3′ 4′ 5′ 5 7-五羟黄酮 表没食子儿茶素 

分 类 号：R285.5[医药卫生—中药学]