HPLC-HRMS结合多元统计分析不同提取方法槐米提取物中黄酮类差异性成分  

Differential flavonoid compounds in extracts of Sophorae Flos Immaturus by different extraction methods based on HPLC-HRMS combined with multivariate statistical analysis

作　　者：肖禹圣 常言言 赵孟雅 田璐 李钠 赵幻希 修洋 XIAO Yu-sheng;CHANG Yan-yan;ZHAO Meng-ya;TIAN Lu;LI Na;ZHAO Huan-xi;XIU Yang(Jilin Ginseng Academy,Changchun University of Chinese Medicine,Changchun 130117,China)

机构地区：[1]长春中医药大学吉林省人参科学研究院,长春130117

出　　处：《天然产物研究与开发》2024年第12期2042-2050,共9页Natural Product Research and Development

基　　金：吉林省科技发展计划(YDZJ202401089ZYTS,YDZJ202201ZYTS261,20210204098YY);吉林省教育厅科学研究项目(JJKH20241090KJ)。

摘　　要：考察加热回流和超声辅助提取的槐米中黄酮类化合物的成分和含量差异。利用高效液相色谱-高分辨质谱(high performance liquid chromatography-high resolution mass spectrometry,HPLC-HRMS)技术建立芦丁、烟花苷、水仙苷和槲皮素等4种黄酮类化合物的定性与定量分析方法。以4种化合物的绝对含量为指标优化提取条件,建立优化的加热回流和超声辅助提取方法。采用主成分分析和偏最小二乘-判别分析比较两种方法提取物之间的成分差异。结果显示,4种化合物具有不同的最优提取条件,加热回流和超声辅助提取的4种化合物总含量分别为245.82 mg/g和303.34 mg/g,其中加热回流适合提取槐米中烟花苷和水仙苷,超声辅助适合提取芦丁和槲皮素。两种方法的提取物中23种成分存在显著性差异,鉴定了其中9个差异化合物。研究结果明确了槐米提取物在两种常用提取方法下的差异性,为槐米中黄酮类成分的高效提取提供了有益参考。The differences in the composition and content of flavonoids in Sophorae Flos Immaturus extracted by heating reflux and ultrasonic-assisted extraction were investigated.The qualitative and quantitative analyses of four flavonoids,including rutin,nicotiflorin,narcissoside and quercetin,were established using high performance liquid chromatography-high resolution mass spectrometry(HPLC-HRMS).The heating reflux and ultrasonic-assisted extraction conditions were optimized based on the absolute contents of the four flavonoid compounds,and optimized extraction methods were developed.Principal component analysis and partial least squares-discriminant analysis were employed to compare the composition differences between the extracts obtained by the two extraction methods.The results showed that the four flavonoid compounds had different optimal extraction conditions,their total contents in the heating reflux and ultrasonic-assisted extracts were 245.82 mg/g and 303.34 mg/g,respectively.Significant difference was observed between the extracts of the two extraction methods in 23 components,of which nine differential compounds,were identified.This study clarified the differences in flavonoid composition extracted from Sophorae Flos Immaturus by heating reflux and ultrasonic-assisted extraction methods,providing valuable references for the efficient extraction of flavonoid compounds in Sophorae Flos Immaturus.

关 键 词：槐米 黄酮 提取 高效液相色谱-高分辨质谱 多元统计分析 

分 类 号：R284.1[医药卫生—中药学] O657.63[医药卫生—中医学]