基于UPLC-Q-TOF-MS/MS和HPLC的广东产广地龙鲜、干品主要化学成分分析  

Analysis of the main chemical components in fresh and dried Pheretima aspergillum from Guangdong by UPLC-Q-TOF-MS/MS and HPLC

作　　者：胡小勤 蒙丹[2] 曾学文 廖承谱 赵威 丁雪菲 HU Xiao-qin;MENG Dan;ZENG Xue-wen;LIAO Cheng-pu;ZHAO Wei;DING Xue-fei(Jinhua Advanced Research Institute,Jinhua 321013,China;Guangxi Ecological Engineering Vocational and Technical College,Liuzhou 545004,China;Jinhua Institute of Zhejiang University,Jinhua 321299,China;Guangxi University of Traditional Chinese Medicine,Nanning 530200,China)

机构地区：[1]金华高等研究院,金华321013 [2]广西生态工程职业技术学院,柳州545004 [3]浙江大学金华研究院,金华321299 [4]广西中医药大学,南宁530200

出　　处：《天然产物研究与开发》2024年第12期2051-2063,共13页Natural Product Research and Development

基　　金：首届建生药业鲜药创研基金(JSJC-20190105-047);广西中医药大学2019年广西一流学科建设开放课题(2019XK071)。

摘　　要：比较广东产的鲜、干广地龙化学成分差异,测定广地龙中次黄嘌呤、黄嘌呤、尿嘧啶的含量,建立广地龙质量评价标准。采用超高效液相色谱-飞行时间质谱(UPLC-Q-TOF-MS/MS)技术分析鲜、干广地龙中主要化学成分,高效液相色谱法(HPLC)测定鲜、干广地龙次黄嘌呤、黄嘌呤、尿嘧啶的含量。结果表明,通过文献及实验室建立的数据库比对,干广地龙解析得56个化合物,其中11种游离氨基酸类,16种有机酸类,核苷类9种,9种二肽类及环二肽类,11种含氮类及其他类;鲜广地龙解析得到48个化合物,其中11种游离氨基酸类,17种有机酸类,10种核苷类,8种二肽类及环二肽类,2种含氮类及其他类。次黄嘌呤、黄嘌呤、尿嘧啶分离效果较好,标准曲线在线性范围线性良好(r=0.9999),平均加样回收率在98.36%~99.82%,相对标准偏差在0.25%~3.1%。本实验采用的UPLC-Q-TOF-MS/MS技术为鉴定鲜、干广地龙化学成分提供快速、高效的定性分析方法,HPLC法建立简单且快速、准确地测定次黄嘌呤、黄嘌呤、尿嘧啶定量方法。该研究为鲜、干广地龙的化学成分研究以及质量控制提供科学依据。This study aims to compare the chemical composition differences between fresh and dried Pheretima aspergillum produced in Guangdong and to establish quality evaluation standards by determining the contents of hypoxanthine,xanthine,and uracil.Ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS)was used to analyze the primary chemical components in both fresh and dried samples,while high-performance liquid chromatography(HPLC)was employed to quantify the levels of hypoxanthine,xanthine,and uracil.The results indicated that,based on comparisons with databases established from literature and laboratory work,56 compounds were identified in the dried samples,including 11 free amino acids,16 organic acids,9 nucleosides,9 dipeptides and cyclic dipeptides,and 11 nitrogen-containing and miscellaneous compounds.In the fresh samples,48 compounds were identified,including 11 free amino acids,17 organic acids,10 nucleosides,8 dipeptides and cyclic dipeptides,and 2 nitrogen-containing and miscellaneous compounds.The separation of hypoxanthine,xanthine,and uracil was achieved with satisfactory results,exhibiting good linearity within the calibration range(r=0.9999).The average recovery rates ranging were between 98.36%and 99.82%,and the relative standard deviations were between 0.25%and 3.1%.The UPLC-Q-TOF-MS/MS technique used in this study provided a rapid and efficient qualitative analysis method for identifying the chemical components in both fresh and dried Pheretima aspergillum samples.The HPLC method developed is simple,fast,and accurate for quantifying hypoxanthine,xanthine,and uracil.This research provides a scientific basis for studying the chemical composition and quality control of fresh and dried Pheretima aspergillum.

关 键 词：广地龙 超高液相串联质谱法 高效液相色谱法 鲜品 干品 成分分析 

分 类 号：R284.1[医药卫生—中药学]