短小芽孢杆菌SE5肽聚糖和脂磷壁酸联合添加对斜带石斑鱼免疫、抗氧化和肠道形态的影响  

Effects of combined supplementation of Bacillus pumilus SE5 peptidoglycan and lipoteichoic acid on immune,antioxidant capacity,and intestinal morphology of Epinephelus coioides

作　　者：王奇桢 于海霞 李杰 杨红玲[1,2] 吴卫强 吴子烨 靳婷 孙云章[1,2] WANG Qi-zhen;YU Hai-xia;LI Jie;YANG Hong-ling;WU Wei-qiang;WU Zi-ye;JIN Ting;SUN Yun-zhang

机构地区：[1]集美大学水产学院海水养殖生物育种全国重点实验室,福建厦门361021 [2]集美大学水产学院厦门市饲料检测与安全评价重点实验室,福建厦门361021 [3]厦门昶科生物工程有限公司,福建厦门361000

出　　处：《饲料研究》2024年第21期62-66,共5页Feed Research

基　　金：国家自然科学基金项目(项目编号:32072990);厦门市自然科学基金项目(项目编号:3502Z202373031);福建省农业引导性(重点)项目(项目编号:2023N0012);福建省大学生创新创业训练计划项目(项目编号:202210390063)。

摘　　要：试验旨在探究短小芽孢杆菌SE5提取的肽聚糖、脂磷壁酸联合添加对斜带石斑鱼(Epinephelus coioides)免疫、抗氧化和肠道形态的影响。试验挑选初始体重(14.69±0.04)g的健康石斑鱼540尾,随机分为6组,每组3个重复,每个重复30尾鱼。对照组(CG组)饲喂基础饲料,试验组在基础饲料中分别添加灭活短小芽孢杆菌SE5(1.0×10^(8) CFU/g,HIB组)、肽聚糖(52.86 mg/kg,PGN组)、脂磷壁酸(6.60 mg/kg,LTA组),联合添加肽聚糖和脂磷壁酸(26.43 mg/kg+3.30 mg/kg,PLA01组;52.86 mg/kg+6.60 mg/kg,PLA02组)。试验期8 w。结果显示,HIB组和PLA01组斜带石斑鱼肝脏的酸性磷酸酶(ACP)活性显著高于CG组和LTA组(P<0.05)。HIB组肠道的超氧化物歧化酶(SOD)活性显著高于CG组(P<0.05)。各试验组肠道丙二醛(MDA)含量均显著低于CG组(P<0.05)。除HIB组和LTA组外,各试验组肠道的绒毛高度均显著高于CG组(P<0.05),PLA01组最长;PLA02组肌层厚度最厚,PGN组和PLA02组的肌层厚度均显著高于CG组(P<0.05)。研究表明,联合添加短小芽孢杆菌SE5脂磷壁酸与肽聚糖对斜带石斑鱼免疫功能、肠道抗氧化能力及肠道形态的促进作用优于单独添加,且以PLA01组添加效果较好。The experiment aimed to investigate the effects of peptidoglycan and lipoteichoic acid extracted from Bacillus pumilus SE5,when added individually and in combination,on the immune response,antioxidant capacity,and intestinal morphology of Epinephelus coioides.A total of 540 healthy groupers with an initial body weight of(14.69±0.04)g were randomly divided into six groups,with three replicates per group and 30 fish per replicate.The control group(CG group)was fed a basal diet,while the experimental groups were fed the basal diet supplemented with inactivated Bacillus pumilus SE5(1.0×10^(8) CFU/g,HIB group),peptidoglycan(52.86 mg/kg,PGN group),lipoteichoic acid(6.60 mg/kg,LTA group),and combined peptidoglycan and lipoteichoic acid(26.43 mg/kg+3.30 mg/kg,PLA01 group;52.86 mg/kg+6.60 mg/kg,PLA02 group).The trial period was eight weeks.The results showed that the acid phosphatase(ACP)activity in the liver of Epinephelus coioides in the HIB and PLA01 groups was significantly higher than that in the CG and LTA groups(P<0.05).The intestinal superoxide dismutase(SOD)activity in the HIB group was significantly higher than that in the CG group(P<0.05).The malondialdehyde(MDA)content in the intestines of all experimental groups was significantly lower than that in the CG group(P<0.05).Except for the HIB and LTA groups,the villus height in the intestines of all experimental groups was significantly higher than that in the CG group(P<0.05),with the PLA01 group having the longest villi.The muscle layer thickness was greatest in the PLA02 group,and the muscle layer thickness in the PGN,PLA02 groups was significantly higher than that in the CG group(P<0.05).The study indicates that the combined addition of lipoteichoic acid and peptidoglycan from Bacillus pumilus SE5 has a better promoting effect on the immune efficiency,intestinal antioxidant capacity,and intestinal morphology of Epinephelus coioides than individual additions,and PLA01 group shows better potential.

关 键 词：斜带石斑鱼 短小芽孢杆菌SE5 肽聚糖 脂磷壁酸 免疫功能 

分 类 号：S963[农业科学—水产养殖]