枸杞多糖对阿司匹林诱导小鼠肠道损伤的保护作用  

Protective effect of Lycium barbarum polysaccharide on aspirin-induced intestinal injury in mice

作　　者：甘海清 陈佳亿 夏艳菊 肖青云 刘蓉蓉 李宏坤 谢仁杰 黄兴国[1] GAN Hai-qing;CHEN Jia-yi;XIA Yan-ju

机构地区：[1]湖南农业大学动物科学技术学院,湖南长沙410128 [2]长沙医学院中医药农业生物基因组学湖南省重点实验室,湖南长沙410219

出　　处：《饲料研究》2024年第21期80-84,共5页Feed Research

基　　金：湖南省教育厅一般课题(项目编号:22C0674);长沙医学院ESI学科建设重点项目(项目编号:2022CYY006)。

摘　　要：试验旨在研究枸杞多糖(LBP)对阿司匹林(ASA)诱导小鼠肠道损伤的保护作用。选取24只7周龄的雄性昆明小鼠,适应性饲养7 d后随机分为对照组、阿司匹林组(ASA组)和枸杞多糖组(LBP组),每组8只,试验期12 d。试验期前6 d对照组灌胃200 mg/kg生理盐水,ASA组和LBP组灌胃等量阿司匹林;试验期后6 d LBP组灌胃75 mg/kg枸杞多糖,对照组和ASA组灌胃等量生理盐水。结果显示,与对照组相比,ASA组的空肠绒毛高度(VH)显著降低(P<0.05),绒毛高度/隐窝深度(VH/CD)极显著降低(P<0.01);回肠超氧化物歧化酶(SOD)活性极显著降低(P<0.01),空肠和回肠丙二醛(MDA)含量极显著提高(P<0.01)。与ASA组相比,LBP组肾脏指数显著降低(P<0.05),空肠VH/CD、空肠和回肠的SOD活性极显著提高(P<0.01),空肠和回肠MDA含量极显著降低(P<0.01)。结果表明,枸杞多糖可增强阿司匹林诱导小鼠损伤的空肠和回肠抗氧化能力,改善肠道形态结构,缓解小鼠肠道损伤。The experiment aimed to investigate the protective effects of Lycium barbarum polysaccharides(LBP)on aspirin(ASA)-induced intestinal injury in mice.The twenty-four 7-week-old male Kunming mice were randomly divided into a control group,aspirin model group(ASA group),and Lycium barbarum polysaccharide group(LBP group)after seven days of adaptive feeding,with eight mice in each group.The experimental period was 12 days.In the first six days of the experiment,the control group was gavage 200 mg/kg normal saline,and the ASA group and the LBP group were gavage the same amount of aspirin.On the last six days of the experiment,the LBP group was gavage 75 mg/kg LBP,and the control group and ASA group were gavage the same amount of normal saline.The results showed that compared with the control group,the jejunal villus height(VH)of the ASA group was significantly decreased(P<0.05)and villus height/crypt depth(VH/CD)was extremely decreased(P<0.01),the activity of superoxide dismutase(SOD)in the ileum was extremely decreased(P<0.01),the content of malondialdehyde(MDA)in the jejunum and ileum was extremely increased(P<0.01).Compared with the ASA group,the LBP group significantly decreased kidney index(P<0.05),extremely increased VH/CD in jejunum and SOD activity in jejunum and ileum(P<0.01),and extremely decreased MDA content in jejunum and ileum(P<0.01).The study indicates that LBP can enhance the antioxidant capacity of jejunum and ileum in ASA-induced intestinal injury in mice,improve intestinal morphology,and alleviate intestinal injury in mice.

关 键 词：枸杞多糖 阿司匹林 肠道损伤 肠道形态结构 抗氧化 小鼠 

分 类 号：S816.7[农业科学—饲料科学]