Practical applications of total internal reflection fluorescence microscopy for nanocatalysis  

作　　者：Chengyang Yan Xuanhao Mei Xue Gong Weilin Xu 

机构地区：[1]State Key Laboratory of Electroanalytical Chemistry,Jilin Provincial Key Laboratory of Low Carbon Chemical Power,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China [2]University of Science and Technology of China,Hefei 230026,China

出　　处：《Industrial Chemistry & Materials》2024年第1期85-99,共15页工业化学与材料（英文）

基　　金：This work was supported by the National Science Foundation of China(21925205,22072145,22102172,21721003)。

摘　　要：Fluorescence microscopy has evolved from a purely biological tool to a powerful chemical instrument for imaging and kinetics research into nanocatalysis.And the demand for high signal-to-noise ratio and temporal–spatial resolution detection has encouraged rapid growth in total internal reflection fluorescence microscopy(TIRFM).By producing an evanescent wave on the glass–water interface,excitation can be limited to a thin plane to ensure the measured accuracy of kinetics and image contrast of TIRFM.Thus,this unique physical principle of TIRFM makes it suitable for chemical research.This review outlines applications of TIRFM in the field of chemistry,including imaging and kinetics research.Hence,this review could provide guidance for beginners employing TIRFM to solve current challenges creatively in chemistry.

关 键 词：Total internal reflection fluorescence microscopy NANOCATALYSIS IMAGING Kinetics analysis 

分 类 号：O64[理学—物理化学]