Yak bone collagen-derived anti-inflammatory bioactive peptides alleviate lipopolysaccharide-induced inflammatory by inhibiting the NF-κB signaling pathway and nitric oxide production  

作　　者：Yuliang Yang Lingyu Zhu Zitao Guo Chunyu Liu Bo Hu Moying Li Zhenghua Gu Yu Xin Haiyan Sun Yanming Guan Liang Zhang 

机构地区：[1]National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing,Jiangnan University,Wuxi,214122,China [2]Jiangsu Provincial Research Center for Bioactive Product Processing Technology,Jiangnan University,Wuxi,214122,China [3]National Engineering Research Center for Functional Food,Jiangnan University,Wuxi,214122,China [4]Hainan Key Laboratory of Tropical Microbe Resources,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,571101,China [5]China National Research Institute of Food and Fermentation Industries Co.,Ltd.,Beijing,100015,China

出　　处：《Food Bioscience》2023年第2期825-833,共9页食品生物科学（英文）

基　　金：This work was supported by Postdoctoral Science Foundation of Jiangsu Province(2021K269B);the National Key Research&Developmental Program of China(2021YFC2100300);China Agriculture Research System of Finance and Agriculture Ministry(No.CARS-11-HNSHY);Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences(No.1630052021012,1630052020023,1630052020024).

摘　　要：Anti-inflammatory peptides that derived from food attract more and more attention due to their wide range of sources and easy absorption.In this study,the aim was to identify and characterize anti-inflammatory peptides from yak bone collagen.The prepared yak bone collagen peptides(YBCPs)was separated,purified and identified through ultrafiltration,reverse-phase high-performance liquid chromatography(RP-HPLC),and nano-liquid chromatography-tandem mass spectrometry(nano LC-MS/MS)successively.Finally,a total of 115 peptides were identified.After that,12 peptides were screened out based on the predicted biological activity score.Molecular docking results indicated that 6 peptides(GPAGPSGPAGK,GPAGPSGPAGKDGR,GPSGPQGIR,GPAGPQGPR,GEAGPAGPAGPAGPR,and GEGGPQGPR)could successfully interact with the key factors in the nuclear factor kappa-B(NF-κB)signaling pathway and nitric oxide(NO)production due to form various bonds such as salt bridge,conventional hydrogen bond,and carbon-hydrogen bond.The anti-inflammatory effects of these 6 peptides were further verified by in vitro cell test.Results indicated that the 6 peptides might play an anti-inflammatory role by regulating different proteins in the NF-κB signaling pathway and NO production.This study would further promote the application of yak bone collagen in health food.

关 键 词：Yak bone collagen Anti-inflammatory peptides Separation and purification In silico analysis Western blot 

分 类 号：TS251[轻工技术与工程—农产品加工及贮藏工程]