Characterization of three African swine fever viruses from diferent clinical settings revealed a potential attenuation mechanism  

作　　者：Jiajia Zhang Yuening Wang Kaili Zhang Dafu Deng Kai Peng Wanglong Zheng Nanhua Chen Penggang Liu Shaobin Shang Ruichao Li Jianzhong Zhu 

机构地区：[1]College Veterinary Medicine,Yangzhou University,Yangzhou,China [2]Joint International Research Laboratory of Agriculture and Agri‑Product Safety,Yangzhou University,Yangzhou,China [3]Comparative Medicine Research Institute,Yangzhou University,Yangzhou,China [4]Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou University,Yangzhou 225009,China

出　　处：《Animal Diseases》2024年第4期263-274,共12页动物疾病（英文）

基　　金：supported by the National Key Research and Develop‑ment Program of China under Grant[2021YFD1800105];the Jiangsu Provincial Key R&D Plan under Grant[BE2020398];the Jiangsu Agricultural Science and Technology Independent Innovation Fund Project under Grant[CX(21)2035];the 111 Project under Grant D18007;the A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD);supported by Research and Practice Innovation Project of Jiangsu Province Graduate Students(SJCX24_2310).

摘　　要：African swine fever(ASF)is an acute and fatal hemorrhagic disease in domestic pigs and wild boars caused by African swine fever virus(ASFV)that currently threatens the pig industry worldwide.Since the 2018 ASF outbreak in China,ASFV has evolved and caused diverse clinical manifestations,such as chronic and asymptomatic infections.Therefore,it is important to understand the molecular mechanisms underlying ASFV attenuation in the feld.Here,we isolated three ASFVs from one diseased and two asymptomatic pigs by using primary porcine alveolar macrophages(PAMs)from both domestic pigs and Bama minipigs.The three ASFVs exhibited similar phenotypes in cell culture,includ‑ing cytopathic efects(CPEs),hemadsorptions(HADs),viral protein expressions and growth curves.Genome sequenc‑ing revealed that all three ASFVs were genotype II strains.Genomic comparisons suggested that the disruption of the viral genes MGF360 and MGF110,rather than EP402R and EP153R,is likely involved in the potential attenuation of ASFV via the upregulation of innate immune responses.

关 键 词：African swine fever virus Virus isolation Genome sequence Genotype II ATTENUATION 

分 类 号：S852.65[农业科学—基础兽医学]