Diverse immune cell profles in ASFV-associated lymphopenia  

作　　者：Wenjing Xiong Haowei Chen Yanru Chen Ke Wang Tingting Lian Weijia Zhang Qing Yu Xiaochen Gao Jie Su Qigai He Xiangru Wang Junping Yu Min Cui 

机构地区：[1]State Key Laboratory of Agricultural Microbiology,College of Veterinary Medicine,Huazhong Agricultural University,Hongshan District,No.1,Shizishan Street,Wuhan 430070,China [2]Key Laboratory of Preventive Veterinary Medicine in Hubei Province,The Cooperative Innovation Centre for Sustainable Pig Production,Wuhan,China [3]Key Laboratory of Development of Veterinary Diagnostic Products,Ministry ofAgriculture of the People’s Republic of China,Wuhan,China [4]International Research Centre for Animal Disease,Ministry of Science and Technology of the People’s Republic of China,Wuhan,China [5]Key Laboratory of Special Pathogens and Biosafety,Centre for Emerging Infectious Diseases,Wuhan Institute of Virology,Chinese Academy of Sciences,No 262,Gold Science&Technology Park,Jinlong Street,Hubei Province,Wuhan 430207,China

出　　处：《Animal Diseases》2024年第4期275-288,共14页动物疾病（英文）

基　　金：support provided by the National Natural Sciences Foundation of China(Grant Nos.32172873 and 31941005);the National Programme on Key Research Project of China(2021YFD1800105-2);National Natural Sciences Foundation of China,32172873,Min Cui,31941005,Min Cui,National Programme on Key Research Project of China,2021YFD1800105-2,Min Cui。

摘　　要：Pathogenic African swine fever virus(ASFV)remains a lethal causative agent in the domestic pig industry,which poses a burden on the swine market and causes substantial socioeconomic losses worldwide.Currently,there are no commercially efcacious vaccines or specifc treatments available for ASF prevention and control.Unfortunately,little is known about the swine immune response upon ASFV infection.Here,we investigated the host immune response discrepancy induced by the feld moderately virulent strain ASFV HB-2208 among healthy,diseased and asymptomatic pigs.In the peripheral blood of diseased swine,lymphopenia is caused by the massive loss of bystander lymphocytes,such asγδT cells,B cells and CD4^(+)T cells.Conversely,ASFV has a strong tropism for the mononuclear phagocyte system(MPS)and partial dendritic cells(DCs),whose antigen-presenting ability is impeded by the downregulation of CD80 and MHC I.However,no signifcant diference in the number of CD8α^(high) T cells was detected,whereas the frequencies of NK cells,NKT cells,and regulatory T cells(Tregs)were signifcantly increased.Additionally,an in vitro model was established with a coculture of primary pulmonary alveolar macrophages(PAMs)and peripheral blood mononuclear cells(PBMCs),which signifcantly reducedγδT cells,B cells and CD4^(+)T cells and increased Tregs.The diferentiated immune response might aid in enhancing the understanding of ASFV pathogenesis in suids and provide insights into the mechanism of ASFV-induced lymphopenia for further studies.

关 键 词：African swine fever virus Antigen-presenting cells LYMPHOCYTES LYMPHOPENIA 

分 类 号：S852.651[农业科学—基础兽医学]