Mutations in the WUSCHEL-related homeobox1 gene cause an increased leaflet number in soybean  

作　　者：Yawei Li Li Chu Peiyun Lyu Wenhuan Lyu Ping Xie Chunting Zhang Haoxian Feng Bin Liu Tuanjie Zhao 

机构地区：[1]Key Laboratory of Eology and Genetis Improverment of Soybear,Ministry of Agriculure/Zhonghur Biological Deeditg Labor atory(乙BBL)/Narionol hnnowation Playform for Soybeat Breedirg and Industry-Education Integrution/Slate Key Laboratory of Crop Generics&Germplasm Ethancement and Uuilzation/College of Agriculure,Nanjirg Agr iculmural Uhtiversity,Nanjing 210095,Jingu,China [2]State Key Laboratory of Crop Gene Resources and Breeding,National Key Facility for Crop Gene Resources and Genetic Improvement,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China

出　　处：《The Crop Journal》2024年第6期1645-1654,共10页作物学报（英文版）

基　　金：supported by the Major Project of National Agricultural Science and Technology of China,the National Natural Science Foundation of China (32171965,32072091,31271750);the Core Technology Development for Breeding Program of Jiangsu Province (JBGS-2021-014);Collaborative Innovation Center for Modern Crop Production Co-sponsored by Province and Ministry (CIC-MCP)Program.

摘　　要：WUSCHEL-related homeobox(WOX)transcription factors play a crucial role in lateral organ development in several plant species;however,their precise functions in soybean(Glycine max[L.]Merr.)were unclear.Here,we identified two independent multi-leaflet soybean mutants,mlw48-8 and mlw48-161,from a CRISPR/Cas9-engineered mutant library in the Williams 82 background.Both mutants exhibited irregular leaf margins,and the upper leaves were narrow and almost lanceolate at maturity.Molecular analysis revealed that these are allelic mutants with independent mutations in the WUSCHEL-related homeobox1(GmWOX1A)gene.A transcriptome analysis demonstrated that GmWOX1A modulates the expression of auxin-and leaf development–related genes.Yeast two-hybrid and split-luciferase complementation imaging assays revealed that GmWOX1A interacts with the YABBY family protein GmYAB5,providing further evidence of its potential involvement in leaf development.Notably,the mlw48-161 mutant showed an increased seed number per plant.Consequently,our study not only provides valuable insights into the role of GmWOX1A in soybean leaf development but also offers a potential strategy for high-yield breeding.

关 键 词：SOYBEAN GmWOX1A GmYAB5 Compound leaf development Seed number per plant 

分 类 号：S565.1[农业科学—作物学]