Gene editing and overexpression of soybean miR396a reveals its role in salinity tolerance and development  

作　　者：Xiangqian Chen Xuemin Jiang Xianjun Sun Zheng Hu Fei Gao Xiuping Wang Hui Zhang Rui Chen Qiyan Jiang 

机构地区：[1]State Key Laboratory of Crop Gene Resources and Breeding,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China [2]Key Laboratory of Grain Crop Genetic Resources Evaluation and Utlization Ministry of Agriculture and Rural Affairs,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China [3]Institute of Crop Germplasm and Biotechnology,Tianjin Academy of Agricultural Sciences,Tianjin 300192,China [4]National Nanfan Research Institute,Chinese Academy of Agricultural Sciences,Sanya 572000,Hainan,China [5]College of Life and Environmental Sciences,Minzu University of China,Beijing 100081,China [6]Institute of Coastal Agriculture,Hebei Academy of Agriculture and Forestry Science,Tangshan 063299,Hebei,China

出　　处：《The Crop Journal》2024年第6期1655-1665,共11页作物学报（英文版）

基　　金：supported by the National Key Research and Development Program of China (2021YFD1201603,2023YFD1202900);the Key Research and Development Program of Shandong Province (Agricultural Seed Improvement Project) (ZDYF2023LZGC001);the Key Research and Development Program of Hainan Province (ZDYF2022XDNY135);the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (ASTIP No.CAAS-ZDRW202407,01-ICS-02).

摘　　要：MicroRNAs(miRNAs)are versatile regulators of gene expression at both the transcription and posttranscription levels.The microRNA miR396 plays vital roles in growth,development,and resistance to abiotic stresses in many plant species.However,the roles and functions of miR396 in soybeans are not well understood.Here,we report that Gm-miR396a influences soybean development and salinity tolerance.We found that soybean miR396a was responsive to salt stress.Gm-miR396a gene-edited lines(miR396a-GEs),created using CRISPR/Cas9,exhibited more branches,higher grain yields,and greater salinity tolerance than control plants.The transcripts in lines with altered abundance of miR396a-GE were significantly enriched for biological processes related to hormone regulation.Overexpression of the Gm-miR396a precursor(pre-miR396a-OE)resulted in developmental deficiencies including dwarfness,abnormal inflorescences and flowers,smaller and fewer seeds,and small leaves with larger and more numerous stomata.Transcriptome analysis indicated photosynthesis-related genes were downregulated in pre-miR396a-OE plants.These results contribute valuable insights into the function of GmmiR396a in soybeans and hold promise for enhancing soybean yield and salinity tolerance through germplasm innovation.

关 键 词：Glycine max Gm-miR396a Salt stress CRISPR/Cas9 

分 类 号：S565.1[农业科学—作物学]