Identification and transferring of a new Fusarium head blight resistance gene FhbRc2 from Roegneria ciliaris 3ScL chromosome arm into common wheat  

作　　者：Rongrong Song Danhua Zhang Jingxin Yang Yifan Cheng Xinying Song Weiping Zhao Mengshuang Xia Yao Zhang Luyang Wei Menghao Cheng Wei Wang Li Sun Haiyan Wang Xiu’e Wang Jin Xiao 

机构地区：[1]National Key Laboratory of Crop Genetics&Germplasm Enhancement and Utilization,College of Agriculture,Nanjing Agricultural University,Zhongshan Biological Breeding Laboratory,Collaborative Innovation Center for Modern Crop Production(CIC-MCP),Nanjing 210095,Jiangsu,China

出　　处：《The Crop Journal》2024年第6期1718-1726,共9页作物学报（英文版）

基　　金：supported by Science and Technology Innovation 2030-Major Project (2023ZD04025);the Fundamental Research Funds for the Central Universities (XUEKEN2022012,YDZX202301);Zhongshan Biological Breeding Laboratory (ZSBBL)ZSBBL-KY2023-02-2,Jiangsu Provincial Key Research and Development Program (BE2022346,BE2021375);Seed Industry Revitalization Project of Jiangsu Province (JBGS (2021)006,JBGS (2021)013 and JBGS (2021)047);the Joint Research of Improved Wheat Variety of Anhui,the Key Research and the Jiangsu Agricultural Technology System (JATS) ([2023]422);Natural Science Foundation of Shanghai (22ZR1444900).

摘　　要：Fusarium head blight(FHB)threatens wheat production worldwide.Utilization of FHB resistant varieties is the most effective solution for disease control.Owing to the limited sources of FHB resistance,mining of novel resistance genes is crucial.Here,we report an FHB resistance gene from a wild wheat relative species,Roegneria ciliaris and developed FHB resistant germplasm containing this gene.Wheat-R.ciliaris disomic addition line DA3S^(c) showed enhanced type II FHB resistance compared to its sister line 3S^(c)-Null without chromosome 3S^(c),indicating that the resistance was contributed by the addition of 3S^(c).The resistance gene on 3S^(c) was validated using F_(2) and F_(2:3) populations derived from the cross between DA3S^(c) and susceptible Aikang 58(a susceptible cultivar),demonstrating that the lines with 3S^(c) had significantly enhanced FHB resistance compared to the individuals without 3S^(c).This was the second resistance gene identified in R.ciliaris,designated FhbRc2.To transfer FhbRc2 to common wheat,we produced a doublemonosomic chromosome population by crossing DA3S^(c) with the Chinese Spring nulli-tetrasomic line N3DT3B.Eight alien chromosome lines containing 3S^(c) were identified using genomic/fluorescence in situ hybridization and 3S^(c)-specific marker analysis.Only the lines carrying the long arm of 3S^(c) conferred FHB resistance,further locating FhbRc2 on 3S^(c)L.A compensating wheat-R.ciliaris Robertsonian translocation line T3DS·3S^(c)L harboring FhbRc2 is developed and provides a potential genetic resource in wheat breeding for enhanced FHB resistance.

关 键 词：Triticum aestivum L. Fusarium head blight Roegneria ciliaris Genomic/Fluorescence in situ hybridization Alien chromosome lines 

分 类 号：S512.1[农业科学—作物学]