Characterization of novel wheat-Thinopyrum ponticum introgression lines derived from partial amphiploid AUS6770 for resistance to stripe rust  

作　　者：Chengzhi Jiang Yujie Luo Yile Qi Senmiao Liu Ian Dundas Guangrong Li Zujun Yang 

机构地区：[1]School of Life Science and Technology,University of Electronic Science and Technology of China,Chengdu 610054,Sichuan,China [2]School of Agriculture,Food and Wine,the University of Adelaide,Waite Campus,Glen Osmond,SA 5064,Australia

出　　处：《The Crop Journal》2024年第6期1735-1744,共10页作物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (31971886);International Cooperation Program Program (2022YFH0012)of the Science and Technology Department of Sichuan.

摘　　要：The wild decaploid species Thinopyrum ponticum(Podp.)Barkworth&D.R.Dewey is an important source of genes against biotic and abiotic stresses affecting wheat.The wheat–Th.ponticum partial amphiploid AUS6770 shows resistance to multiple diseases,including stripe rust,stem rust,and powdery mildew.Mitotic chromosomes of AUS6770 were characterized by non-denaturing-fluorescence in situ hybridization(ND-FISH),and the individual Th.ponticum chromosomes 1Ae to 7Ae were karyotypically distinguished by Oligo-FISH painting using bulked oligo pools based on wheat-barley collinear regions.A novel stripe rust resistant line A155,derived from AUS6770,was found to have 44 chromosomes,including a pair of 2Ae chromosomes and a pair of 6B-6Ae translocations.To detect plants with transfer of resistance genes from A155 to wheat chromosomes,1770 plants were developed from F2–F5 progenies of A155 crossed with the susceptible wheat cultivar MY11 and characterized with ND-FISH using multiple probes.A high frequency of transmission of chromosome 2Ae was observed,and 31 types of 2Ae chromosomal aberrations were identified using ND-FISH.Ten chromosomal bins on the 2Ae chromosome were determined from the deletion and translocation lines based on genome-based PCR markers.In combination with the evaluation of disease resistance,the gene(s)for stripe rust resistance was located on the FL0.79–1.00 of 2AeS and covers the corresponding region of 0–58.26 Mb in the reference genome of Th.elongatum.The newly identified wheat-Th.ponticum 2Ae translocation lines can be exploited as potential germplasm in wheat breeding for stripe rust resistance.

关 键 词：Thinopyrum ponticum ND-FISH Oligo-FISH painting Stripe rust resistance 

分 类 号：S512.1[农业科学—作物学]