Development of the A&GBE base editor for efficiently generating abundant genetic variation in rice  

作　　者：Wenhui Zhang Yaqi Xu Jingxuan Cui Yingshuang Xue Lijie Zhang Wenjuan Wu Chong Wang Hui Zhang 

机构地区：[1]Shanghai Collaborative Innovation Center of Plant Germplasm Resources Development,Shanghai Key Laboratory of Plant Molecular Sciences,College of Life Sciences,Shanghai Normal University,Shanghai 200234,China

出　　处：《The Crop Journal》2024年第6期1801-1805,共5页作物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (32170547)to Hui Zhang;the Program for Professor of Special Appointment (Eastern Scholar)at Shanghai Institutions of Higher Learning (TP2018066)to Hui Zhang;the Natural Science Foundation of Shanghai (21ZR1447100)to Wenjuan Wu.

摘　　要：We have developed a dual base editor,rA&GBE,by fusing adenine and glycosylase base editors.It can induce up to eight types of mutations in T0-generation rice,including single-base conversion,simultaneous multiple-base conversions,and InDels,using a single guide RNA.A-to-G and C-to-G/T conversions occur simultaneously on the same DNA strand.The rA&GBE system may prove useful for crop improvement and in planta direct evolution.

关 键 词：CRISPR A&GBE Genetic variation RICE 

分 类 号：S511[农业科学—作物学]