果寡糖对三株有益菌和三株致病菌体外生长繁殖的影响  

Effects of Fructooligosaccharides on Growth and Reproduction of Three Beneficial Bacteria and Three Pathogenic Bacteria

作　　者：苗颖 杨明凡[2] 李琰[2] 吴非凡 王晶[2] 常伟辰 张红英[2] 张素梅[2] 蔡永超 MIAO Ying;YANG Mingfan;LI Yan;WU Feifan;WANG Jing;CHANG Weichen;ZHANG Hongying;ZHANG Sumei;CAI Yongchao(School of Mathematics and Information Science,Henan University of Economic and Law,Henan Zhengzhou 450046,China;College of Animal Medicine,Henan Agricultural University,Henan Zhengzhou 450046,China;Henan Provincial Animal Quarantine Station,Henan Zhengzhou 450008,China)

机构地区：[1]河南财经政法大学数学与信息科学学院,河南郑州450046 [2]河南农业大学动物医学院,河南郑州450046 [3]河南省动物检疫总站,河南郑州450008

出　　处：《饲料工业》2024年第24期122-127,共6页Feed Industry

基　　金：河南省重点研发项目[221111111300];河南省重点研发专项[231111111600]。

摘　　要：为了探究添加不同比例的果寡糖对三株有益菌(枯草芽孢杆菌、干酪乳杆菌、酿酒酵母菌)和三株致病菌(肠炎沙门氏菌、产肠毒素大肠杆菌、金黄色葡萄球菌)体外生长繁殖的影响。试验采用添加比例为0.5%、1.0%、2.0%的果寡糖培养基分别对以上6种菌株进行体外培养,另设一组不添加果寡糖的培养基组进行对比试验,在培养6、9、15、20、24 h时分别取样测定其OD值。试验结果表明,添加不同浓度(0.5%、1.0%、2.0%)的果寡糖对三株益生菌的生长繁殖有明显促进作用,培养至细菌平衡期时与对照组相比均差异极显著(P<0.01)。对金黄色葡萄球菌的生长繁殖有明显抑制作用,与对照组相比均差异极显著(P<0.01);添加0.5%浓度的果寡糖对产毒素大肠杆菌、肠炎沙门氏菌的生长繁殖有一定的促进作用,但是添加1.0%和2.0%浓度的果寡糖在培养15、20、24 h时对产毒素大肠杆菌、肠炎沙门氏菌的生长繁殖有明显抑制作用,与对照组相比差异极显著(P<0.01)。说明果寡糖作为一种益生元,在体外培养过程中能够促进这三株有益菌的增殖,浓度较高时能够抑制这三株致病菌的生长。This experiment was conducted to study the effect of fructooligosaccharides added in different proportions on the growth and reproduction of three beneficial bacterium(Bacillus subtilis,Lactobacillus casei,Saccharomyces cerevisia)and three pathogenic bacterium(Salmonella enterica,Enterotoxin Esch⁃erichia coli,Staphylococcus aureus).In this experiment,the above six strains were cultured in vitro by adding 0.5%,1.0%and 2.0%fructooligosaccharides culture media.Another group of medium group without fructooligosaccharides was set for comparative test.Taking samples and measuring their OD values at 6 h,9 h,15 h,20 h,and 24 h of cultivation the test results showed that the addition of fructooligosaccharides with different concentrations(0.5%,1.0%,2.0%)significantly promoted the growth and reproduction of three beneficial bacterium,which were significantly different from the control group(P<0.01).Fructooligosaccharides significantly inhibited the growth and reproduction of Staphylococcus aureus,which was extremely significantly different from the control group(P<0.01)when cultured until the bacterial equilibrium period.Adding 0.5%concentration of fructooligosaccharides can promote the growth and reproduction of Enterotoxin Escherichia coli and Staphylococcus aureus.However,the addition of 1.0%and 2.0%concentration of Fructooligosaccharides significantly inhibited the growth and reproduction of Enterotoxin Escherichia coli and Staphylococcus aureus,which was extremely significantly different from the control group(P<0.01).It shows that Fructooligosaccharides,as a prebiotic,can promote the proliferation of these three beneficial bacterium in vitro culture procession,and can inhibit the growth of these three pathogenic bacterium when the concentration is high.

关 键 词：果寡糖 有益菌 致病菌 体外生长繁殖 体外培养 

分 类 号：S816.3[农业科学—饲料科学]