基于16S rDNA序列的秋茄内生细菌多样性分析  

Diversity Analysis of Endophytic Bacteria in Kandelia candel Based on 16S rDNA Sequence

作　　者：陈维峰 林文波 郭萍萍 张春 王思凡 刘芳[2] 袁宗胜 CHEN Wei-feng;LIN Wen-bo;GUO Ping-ping;ZHANG Chun;WANG Si-fan;LIU Fang;YUAN Zong-sheng(College of Geography and Oceanography,Minjiang University,Fuzhou,Fujian 350108,China;College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Fujian Minjiang Estuary Wetland National Nature Reserve Management Office,Fuzhou,Fujian 350200,China;Zhangzhou Forestry Science and Technology Promotion Station,Zhangzhou,Fujian 363000,China;College of Future Technology,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]闽江学院地理与海洋学院,福建福州350108 [2]福建农林大学生命科学学院,福建福州350002 [3]福建闽江河口湿地国家级自然保护区管理处,福建福州350200 [4]漳州市林业科技推广站,福建漳州363000 [5]福建农林大学未来技术学院,福建福州350002

出　　处：《福建农业科技》2024年第10期1-8,共8页Fujian Agricultural Science and Technology

基　　金：福州市林业科技项目(2023RLKY02);福州市林业科技项目(2024RLKY03)。

摘　　要：分析闽江河口湿地秋茄根、枝和叶中可培养内生细菌的多样性,为秋茄内生细菌群落结构、分布、稳定性以及功能内生细菌的研究提供支撑。从秋茄Kandelia candel不同组织中分离可培养内生细菌,基于16S rDNA基因序列比对和系统发育分析内生细菌多样性。结果表明,从秋茄3个组织中分离出36株可培养内生细菌,分属于11属22种(其中2株未被正式命名),其中芽孢杆菌属Bacillus和弧菌属Vibrio为优势菌属,分别占总株数的21.2%和19.4%。不同组织中内生细菌的分布和多样性存在差异,根中内生细菌数量最多(27株,占总株数的75%),枝次之(6株,占总株数的16.7%),叶最少(3株,占总株数的8.3%)。秋茄内生细菌具有丰富的物种多样性,不同组织之间呈现出差异性,根中内生细菌多样性为最丰富。The diversity of culturable endophytic bacteria in the roots,branches and leaves of Kandelia candel in the wetland of Minjiang River estuary was analyzed,in order to provide support for the study of endophytic bacteria community structure,distribution,stability and functional endophytic bacteria in Kandelia candel.In this study,the culturable endophytic bacteria were isolated from different tissues of Kandelia candel,and the diversity of endophytic bacterial was analyzed based on 16S rDNA gene sequence alignment and phylogeny.The results showed that 36 strains of culturable endophytic bacteria were isolated from three tissues of Kandelia candel,belonging to 11 genera and 22 species(2 strains among which were not officially named).Bacillus and Vibrio were the dominant genera,accounting for 21.2%and 19.4%of the total strains,respectively.There were differences in the distribution and diversity of endophytic bacteria in different tissues,among which the number of endophytic bacteria was the highest in roots(27 strains,accounting for 75%of the total number of strains),followed by branches(6 strains,accounting for 16.7%of the total number of strains),and the least in leaves(3 strains,accounting for 8.3%of the total number of strains).The endophytic bacteria in Kandelia candel had rich species diversity in the wetland of Minjiang River estuary,and there were differences among different tissues.The diversity of endophytic bacteria in the roots was the most abundant.

关 键 词：秋茄 内生细菌 分离 多样性 

分 类 号：S345.661[农业科学—作物栽培与耕作技术]