胡杨碱对大鼠急性胃溃疡的预防作用及其机制研究  

The preventive effect and mechanism of Populus euphratican alkaloids on acute gastric ulcer in rats

作　　者：胡大敏 吕伟 孙立稳 马萍 余复昌 赫永强 胡雪飞 HU Damin;LYU Wei;SUN Liwen;MA Ping;YU Fuchang;HE Yongqiang;HU Xuefei(College of Medicine,Tarim University,Alar,Xinjiang 843300;College of Animal Sciences and Technology,Tarim University,Alar,Xinjiang 843300)

机构地区：[1]塔里木大学医学院,新疆阿拉尔843300 [2]塔里木大学动物科学与技术学院,新疆阿拉尔843300

出　　处：《塔里木大学学报》2024年第4期105-112,共8页Journal of Tarim University

基　　金：塔里木大学校长基金硕士人才项目(TDZKSS202104);塔里木大学大学生创新创业训练计划项目(202410757064)。

摘　　要：为探索胡杨碱对大鼠乙醇损伤型急性胃溃疡的保护作用,观察胡杨碱对大鼠超氧化物歧化酶(SOD)活力,丙二醛(MDA)、表皮生长因子(EGF)和血管内皮生长因子(VEGF)含量的影响。将54只SD大鼠随机分为6组:空白组,模型组,阳性对照药组(75 g·L^(-1)雷尼替丁),胡杨碱低浓度组(300μg·mL^(-1))、中浓度组(600μg·mL^(-1))、高浓度组(900μg·mL^(-1)),禁食48 h后,每只大鼠按10 mL·kg^(-1)的量灌胃给予相应组别药物。1.5 h后,除空白组外,其他组灌胃95%乙醇(5 mL·kg^(-1))造模,8 h后测定大鼠急性胃溃疡的面积;观察各组胃组织的HE染色病理学改变;检测大鼠血清中SOD活力和MDA、VEGF、EGF含量。结果表明,与模型组相比,胡杨碱中浓度组大鼠的胃溃疡指数显著降低;SOD活力、EGF含量显著升高;MDA、VEGF含量显著降低。故600μg·mL^(-1)胡杨碱能显著减轻乙醇诱导的急性胃黏膜损伤,对大鼠乙醇诱导的急性胃溃疡具有保护作用。To explore the protective effect of Populus euphratican alkaloids on ethanol-induced acute gastric ulcers in rats,the superoxide dismutase(SOD)activity,malondialdehyde(MDA)content,epidermal growth factor(EGF),and vascular endothelial growth factor(VEGF)levels were observed.A total of 54 SD rats were randomly divided into six groups:a blank control group,a model group,a ranitidine group(75 g·L^(-1)),and low-concentration,medium-concentration,and high-concentration of Populus euphratica alkaloid groups(300μg·mL^(-1),600μg·mL^(-1) and 900μg·mL^(-1),respectively).Following a 48-hour fast,each rat was administered the corresponding group of drugs by gavage at a dose of 10 mL·kg^(-1).After 1.5 hours,with the exception of the control group,95%ethanol(5 mL·kg^(-1))was administered orally to the remaining groups in order to create a model.Following a period of eight hours,the area of acute gastric ulcers in rats was measured.Histological examination using haematoxylin and eosin(HE)staining was then conducted to observe the pathological changes in gastric tissues within each group.The activity of SOD,the levels of MDA,VEGF and EGF in rat serum were examined.The results demonstrated that the gastric ulcer index of rats in the 600μg·mL^(-1) Populus euphratica alkaloid group was significantly reduced in comparison to the model group.Additionally,the activity of SOD and the level of EGF were significantly increased,while the content of MDA and VEGF were significantly decreased.It can therefore be concluded that 600μg·mL^(-1) Populus euphratica alkaloids can significantly alleviate ethanol-induced acute gastric mucosal injury and have a protective effect on ethanol-induced acute gastric ulcers in rats.

关 键 词：胡杨碱 胃溃疡 溃疡抑制率 表皮生长因子 血管内皮生长因子 

分 类 号：R965.1[医药卫生—药理学]