小鼠中央内侧核向前额叶和岛叶皮质发出分支投射的形态学研究  

Morphological study on collateral projections from the central medial thalamic nucleus to the prefrontal cortex and insular cortex in mice

作　　者：薛美琪 窦雪瑛 刘楚涵 王珊 杨彦玲[1] 董玉琳 XUE Meiqi;DOU Xueying;LIU Chuhan;WANG Shan;YANG Yanling;DONG Yulin(Department of Human Anatomy,School of Basic Medical Sciences,Yan'an University,Yan'an 716000,China;Department of Anatomy,Histology and Embryology,K.K.Leung Brain Research Centre,School of Basic Medical Sciences,Air Force Medical University,Xi'an 710032,China)

机构地区：[1]延安大学基础医学院人体解剖学教研室,陕西延安716000 [2]空军军医大学基础医学院人体解剖与组织胚胎学教研室暨梁銶琚脑研究中心,陕西西安710032

出　　处：《空军军医大学学报》2024年第12期1387-1391,1398,共6页Journal of Air Force Medical University

基　　金：国家自然科学基金(32271045);陕西省重点研发计划一般项目(2023-YBSF-156)。

摘　　要：目的观察小鼠丘脑中央内侧核(CM)向不同皮层区域的分支投射情况,并为其参与伤害性信息传递提供形态学证据。方法将荧光金(FG)分别注射到前额叶皮质(PFC)或岛叶皮质(IC)并建立完全弗氏佐剂(CFA)炎性痛模型,观察FG逆标神经元在CM中的分布,以及CM内向PFC或IC投射的神经元是否参与伤害性信息传递;继而将FG注射到PFC,同时将四甲基罗达明(TMR)注入IC,并建立CFA模型,观察CM内FG与TMR双标神经元的分布及其与FOS蛋白的共标。结果(1)将FG注射到PFC后,在CM的吻侧和中部可观察到大量逆标神经元,尾侧的FG逆标神经元相对较少;将FG注射到IC后,可观察到CM的中尾侧有大量的FG逆标神经元分布,而在CM的吻段较少;(2)CM-IC或CM-PFC通路在CFA模型下均可观察到激活的神经元,但前者激活数量较后者多;(3)CM吻中段少量神经元可同时向PFC和IC发出分支投射,且偶见共标神经元在疼痛状态下被激活。结论CM可以分别向PFC和IC发出大量投射,且参与伤害性信息的传递,但分支投射的情况较少见。Objective To observe the collateral projection of the central medial thalamic nucleus(CM)to different cortical regions in mice and to provide morphological evidence for its involvement in the transmission of nociceptive information.Methods Fluoro-Gold(FG)was injected into the prefrontal cortex(PFC)or insular cortex(IC),respectively,and a complete Freund's adjuvant(CFA)model of inflammatory pain was established to observe the distributions of FG retrogradely labeled neurons in CM and whether neurons projecting to PFC or IC in CM were involved in the transmission of nociceptive information.Subsequently,FG was injected into PFC,while tetramethylrhodamine(TMR)was injected into IC,and a CFA model was established to observe the distribution of double-labeled neurons of FG and TMR and their co-labeling with FOS proteins in CM.Results①After injection of FG into PFC,a substantial number of retrogradely labeled neurons were observed in the rostral and medial parts of CM,with a relatively small number of FG retrogradely labeled neurons present in the caudal part.After injection of FG into IC,a considerable number of FG retrogradely labeled neurons were observed in the medial and caudal parts of CM,with less in the rostral part of CM.②Activated neurons were observed in both CM-IC and CM-PFC pathway in CFA model,with a larger number of activated neurons present in the former.③A small number of neurons in the rostral and medial parts of the CM could send collateral projections to both PFC and IC,but co-labeled neurons were occasionally seen to be activated in the pain state.Conclusion CM can send a large number of projections to PFC and IC,respectively,and is involved in the transmission of nociceptive information.However,the collateral projections are less common.

关 键 词：中央内侧核 分支投射 前额叶皮质 岛叶 疼痛 神经通路 

分 类 号：R402[医药卫生—临床医学] R322.81