碳点基纳米酶催化剂用于比色法检测烟叶样品中亚硝酸盐  

Carbon Dots-based Nanozyme Catalyst for Sensitive and Selective Colorimetric Detection of Nitrite

作　　者：何彬 刘辉 蔡冰 段丽 杨艳波 罗净馨 郑娇 张海 He Bin;Liu Hui;Cai Bing;Duan Li;Yang Yanbo;Luo Jingxin;Zhang Jiao;Zhang Hai(Yunnan Tobacco Leaf Company,Kunming 650218,China)

机构地区：[1]云南省烟草烟叶公司,云南昆明650218

出　　处：《云南化工》2024年第12期106-111,共6页Yunnan Chemical Technology

基　　金：中国烟草总公司云南省公司一般项目(2022530000216102)资助。

摘　　要：目的:建立烟叶样品中亚硝酸盐(NO^(-)_(2))纳米酶基比色检测方法。方法:基于铜、碘掺杂碳点(Cu,I-CDs)具有优异的类过氧化物酶(POD)催化活性,在酸性条件及H_(2)O_(2)存在下,可将无色的3,3',5,5'-四甲基联苯胺(TMB)氧化为蓝色的oxTMB,并在654 nm处出现明显的吸收峰。随着亚硝酸盐(NO-2)的加入,基于NO2的形成,体系的类过氧化物酶催化活性得到增强,溶液蓝色加深,建立了一种比色检测亚硝酸盐的新方法。结果:在0.05~17.5 mg/L质量浓度范围内,A_(654)值与NO^(-)_(2)的质量浓度呈良好的线性关系,检测限为0.0126 mg/L(S/N=3)。结论:所构建的检测体系在回收率和抗干扰性方面表现突出,具有较好的检测准确性,样品加标回收率在96.9%以上。该传感器已成功应用于烟叶样品检测,该方法具有快速、简便、稳定、特异性强等特点。(Objective)A nanozymatic colorimetric method for the determination of nitrite(NO^(-)_(2))in tobacco leaf samples was established.(Methods)Based on the excellent peroxidase-like(POD)catalytic activity of copper and iodine doped carbon dots(Cu,I-CDs),colorless 3,3′,5,5′-tetramethylbenzidine(TMB)can be oxidized to blue oxTMB under acidic conditions and in the presence of H_(2)O_(2),and an obvious absorption peak appears at 652 nm.With the addition of nitrite(NO^(-)_(2)),based on the formation of NO_(2),the peroxidase-like catalytic activity of the system was enhanced,and the blue color of the solution was deepened.A new colorimetric method for the detection of nitrite was established.(Results)In the range of 0.05-17.5 mg/L,the A_(654)value showed a good linear relationship with the concentration of NO^(-)_(2),and the detection limit was 0.0126 mg/L(S/N=3).(Conclusion)At the same time,the constructed detection system has outstanding performance in recovery rate and anti-interference,and has good detection accuracy,and the recovery rate of standard addition is above 96.9%.The sensor has been successfully applied to the detection of tobacco leaf samples.The method has the characteristics of fast,simple,stable and strong specificity.

关 键 词：亚硝酸盐 Cu I-CDs 类过氧化物酶 比色检测 快速 烟叶 

分 类 号：TS47[农业科学—烟草工业] O657[理学—分析化学]