HPLC法测定滇紫草及昆明滇紫草中β,β′-二甲基丙烯酰阿卡宁  

Determination ofβ,β′-dimethylacrylylAkanin in Comfrey Yunnanensis and Its Related Variety Kunmingensis by HPLC

作　　者：杨艺妹 郭巍怡 刘晓俊 张羽瑶 Yang Yimei;Guo Weiyi;Liu Xiaojun;Zhang Yuyao(Kunming Food and Drug Inspection Research Institute,Kunming 650000,China)

机构地区：[1]昆明市食品药品检验所,云南昆明650000

出　　处：《云南化工》2024年第12期117-121,共5页Yunnan Chemical Technology

基　　金：2022年云南省市场监督管理局科技计划(2022YSJK05);2023年昆明市科技计划(2023-1-NS-032)。

摘　　要：(目的)建立滇紫草、昆明滇紫草中β,β′-二甲基丙烯酰阿卡宁含量的测定方法。(方法)采用安捷伦液相色谱仪,色谱柱为ThermoAcclaim 120-C_(18)(250 mm×φ4.6 mm,5μm),乙腈-0.2%甲酸(体积比70∶30)等度洗脱,流速1.0 mL/min,柱温30℃,分析波长275 nm。(结果)β,β′-二甲基丙烯酰阿卡宁在24.151~301.889 ng范围内呈良好线性关系(r=0.9998),平均回收率为93.07%,RSD为2.43%(n=6);18批次样品质量分数在0.0934 mg/g~1.7584 mg/g范围内,各批样品质量分数存在差异。(结论)该方法专属性强、重复性好,符合中国药典相关要求,可用于滇紫草及其昆明滇紫草中β,β′-二甲基丙烯酰阿卡宁的含量测定。(Objective)To establish a method for the determination of β,β′-dimethylacrylylarkanin in Comfrey Yunnanensis and Comfrey Kunmingensis.(Methods)The chromatography was performed on a Thermo Acclaim 120-C_(18)(250 mm×∅4.6 mm,5μm)column with isoelution of acetonitrile-0.2%formic acid(70∶30,V/V)at a flow rate of 1.0 mL/min,at a column temperature of 30℃,and at a detection wavelength of 275nm.(Results)β,β′-dimethylacrylylarkanin showed a good linear relationship in the range of 24.151-301.889 ng(r=0.9998),the average recovery was 93.07%,RSD was 2.43%(n=6).The mass fraction of 18 batches of samples was in the range of 0.0934 mg/g-1.7584 mg/g,and the mass fraction of each batch of samples was different.(Conclusion)The method is specific and reproducible,meets the relevant requirements of Chinese Pharmacopoeia.It can be used for the determination of β,β′-dimethylacrylylarkanin in C.yunnanensis and C.kunmingensis.

关 键 词：HPLC 滇紫草 昆明滇紫草 近缘种 β β′-二甲基丙烯酰阿卡宁 含量测定 

分 类 号：R284[医药卫生—中药学] O657[医药卫生—中医学]