Isolation and Identification of Potassium Releasing Bacteria from Sugarcane Rhizosphere Soil and Optimization of Fermentation Condition  

作　　者：Huanhuan JIANG Honghuo HE Cuiting MO Sainan LI Gang CHEN 

机构地区：[1]College of Life Sciences,Zhaoqing University,Zhaoqing 526061,China

出　　处：《Asian Agricultural Research》2024年第12期26-29,共4页亚洲农业研究（英文）

基　　金：Supported by Guangdong Province Rural Science and Technology Commissioner Project(KTP20240693);Zhaoqing University Project(QN202329);Science and Technology Innovation Guidance Project of Zhaoqing(202304038001);Undergraduate Innovation and Entrepreneurship Training Program(202410580011&X202310580120);The Third Batch of Innovation Research Team of Zhaoqing University(05);Quality Engineering and Teaching Reform Project of Zhaoqing University(zlgc202229,zlgc202261).

摘　　要：[Objectives]To isolate and optimize the potassium releasing bacteria from sugarcane rhizosphere soil in Baitu Town,Gaoyao District,Zhaoqing City,Guangdong Province,and then evaluate their potassium-releasing ability,and optimize the fermentation conditions of the strains with the best potassium-releasing ability,so as to provide a scientific basis for the development and production of potassium releasing bacteria fertilizer.[Methods]Potassium-solubilizing bacteria were isolated from sugarcane rhizosphere soil using potassium-feldspar powder as the potassium source of isolation medium by dilution coating method and plate streaking method.The isolated strains were identified by 16s rRNA sequence analysis.The potassium-releasing ability of each strain was determined by sodium tetraphenylborate(STPB)method,and the strain GK-37 with the optimal potassium-releasing ability was selected.The fermentation conditions of GK-37 strain were further optimized,and the effects of different carbon sources on its growth were mainly investigated.[Results]Seven strains of potassium-solubilizing bacteria were isolated and purified,and identified by 16s rRNA.They belonged to Pseudomonas knackmussii,Pseudomonas,Pseudomonas insulaes and Caballeronia zhejiangensis.Among the tested strains,strain GK-37 had the best potassium-releasing ability,and its potassium-releasing capacity was 26.99 mg/L.By optimizing the fermentation conditions of GK-37 strain,it was found that when the fermentation medium was sucrose as carbon source,the growth of the strain was the best.[Conclusions]In this study,the potassium releasing bacteria were successfully isolated and identified from the rhizosphere soil of sugarcane,and the strain GK-37 had high potassium-releasing ability.Through the optimization of fermentation conditions of GK-37 strain,sucrose was determined as the optimal carbon source.This study is expected to provide a valuable reference for the further development and production of potassium releasing bacteria fertilizer.

关 键 词：Potassium releasing bacteria Potassium-feldspar powder Molecular identification Sodium tetraphenylborate Fermentation conditions 

分 类 号：S15[农业科学—土壤学]