高温响应基因MdMYB308抑制苹果花青苷合成  

MdMYB308:High-Temperature-Inducible Gene Suppressing Anthocyanin Accumulation in Apples

作　　者：房月 吴志蒙 刘春帆 徐同尧 李婷 王海波[2] 冯守千 FANG Yue;WU Zhi-meng;LIU Chun-fan;XU Tong-yao;LI Ting;WANG Hai-bo;FENG Shou-qian(College of Horticultural Science and Engineering/Shandong Agricultural University,Tai'an 271018,China;Shandong Institute of Pomology,Tai'an 271000,China)

机构地区：[1]山东农业大学园艺科学与工程学院,山东泰安271018 [2]山东省果树研究所,山东泰安271000

出　　处：《山东农业大学学报（自然科学版）》2024年第6期867-873,共7页Journal of Shandong Agricultural University：Natural Science Edition

基　　金：国家重点研发计划资助(2023YFD2301000);国家自然科学基金项目(31872940)。

摘　　要：高温抑制苹果花青苷合成,从而影响果实的着色。本研究发现高温条件下,MdMYB308基因的表达被强烈诱导。通过亚细胞定位实验,发现MdMYB308定位在细胞核中。为了研究MdMYB308的功能,我们利用过表达和CRISPR/Cas9基因编辑技术对其进行了探究。结果显示,MdMYB308抑制了苹果花青苷结构基因的表达和花青苷合成,MdMYB308在高温抑制苹果花青苷合成中起到了重要的作用,在耐高温红色苹果育种中具有重要的应用潜力。High temperatures suppress anthocyanin synthesis in apples,thereby affecting the fruit's coloration.This study found that the expression of the MdMYB308 gene was strongly induced under high-temperature conditions.Subcellular localization experiments revealed that MdMYB308 is predominantly localized in the cell nucleus.To investigate the function of MdMYB308,we explored it using overexpression and CRISPR/Cas9 gene editing techniques.The results demonstrated that MdMYB308 suppresses the expression of anthocyanin structural genes and anthocyanin synthesis.MdMYB308 plays a significant role in suppressing anthocyanin synthesis under high temperatures and has significant potential for application in breeding heat-tolerant red apples.

关 键 词：苹果 高温 花青苷 MdMYB308 

分 类 号：S661.1[农业科学—果树学]