UHPLC-MS/MS测定睫毛类化妆品中14种前列腺素类似物含量  

Determination of Fourteen Prostaglandin Analogues in Eyelash Cosmetics by UHPLC-MS/MS

作　　者：陈安丽[1] 张静[1] 曾敏珊 严小红[1] 丁怡[1] CHEN Anli;ZHANG Jing;ZENG Minshan;YAN Xiaohong;DING Yi(Guangzhou Institute for Drug Control,Guangzhou 510160,Guangdong,China)

机构地区：[1]广州市药品检验所,广东广州510160

出　　处：《香料香精化妆品》2024年第6期83-89,共7页Flavour Fragrance Cosmetics

基　　金：广州市市场监督管理局科技项目(编号2022kj28)。

摘　　要：建立超高效液相色谱-串联三重四极杆质谱(UHPLC-MS/MS)法测定睫毛类化妆品中苯甲酸拉坦内酯、比马前列素、氯前列醇等14种前列腺素类似物。样品以四氢呋喃为分散剂,经乙腈提取、饱和氯化钠溶液除杂,离心、取上清液过滤后,采用Poroshell 120 EC-C18柱(150 mm×3.0 mm×2.7μm)分离,以5 mmol/L甲酸铵(含质量分数0.1%甲酸)-乙腈为流动相,外标法定量。结果显示,14种前列腺素类似物在5~50 ng/mL线性关系良好,检出限(LOD)均在0.8μg/g内,定量限(LOQ)均在3μg/g以内。平均回收率为81.9%~119.7%,相对标准偏差(RSD)小于5%。该方法快速简便、灵敏准确,适用于睫毛类化妆品中14种前列腺素类似物的测定。A method of ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry(UHPLC-MS/MS)for the determination of 14 prostaglandin analogs,including(-)-corey lactone benzoate,bimatoprost and chloroprostanol in eyelash cosmetics was established in this paper.The samples were extracted with acetonitrile using tetrahydrofuran as the dispersant,decontaminated with saturated sodium chloride solution,centrifuged,and filtered with the supernatant,then separated on a Poroshell 120 EC-C18 column(150 mm×3.0 mm×2.7μm),and quantified by the external standard method with the mobile phase of 5 mmol/L ammonium formate(with a mass fraction of 0.1%formic acid)-acetonitrile.The method showed a good linear relationship in the range of 5 ng/mL and 50 ng/mL,with the limits of detection(LOD)within 0.8μg/g,and the limits of quantitation(LOQ)within 3μg/g.The average recoveries ranged from 81.9%to 119.7%,with the relative standard deviations(RSDs)less than 5%.The method is rapid,simple,sensitive,accurate,and suitable for the determination of 14 prostaglandin analogues in eyelash cosmetics.

关 键 词：睫毛 化妆品 前列腺素类似物 超高效液相色谱-串联三重四极杆质谱法 

分 类 号：TQ658[化学工程—精细化工] O657.63[理学—分析化学]