细胞外ATP受体DORN1对盐胁迫下拟南芥种子萌发的调节作用  

The role of exATP receptor DORN1 in regulating seedgermination of Arabidopsis thaliana under salt stress

作　　者：金昱杉 段建功[2] 王涵琦 贾凌云[1] 孙坤[1] 张继[1,4] 冯汉青[1,4] JIN Yu-shan;DUAN Jian-gong;WANG Han-qi;JIA Ling-yun;SUN Kun;ZHANG Ji;FENG Han-qing(College of Life Sciences,Northwest Normal University,Lanzhou 730070,China;College of Life Sciences,Lanzhou University,Lanzhou 730000,China;Gansu Forestry Vocational and Technical College,Tianshui 741000,Gansu,China;Key Laboratory of Biological Resources Development and Biological Products in the Northwest Plateau,China General Chamber of Commerce,Northwest Normal University,Lanzhou 730070,China)

机构地区：[1]西北师范大学生命科学学院,兰州730070 [2]兰州大学生命科学学院,兰州730000 [3]甘肃林业职业技术学院,天水741000 [4]西北师范大学中国商业联合会西北高原生物资源开发与生物制品重点实验室,兰州730070

出　　处：《兰州大学学报（自然科学版）》2024年第6期759-763,共5页Journal of Lanzhou University(Natural Sciences)

基　　金：国家自然科学基金项目(32460074,31870246);甘肃省自然科学基金重点项目(24JRRA124);甘肃省重点研发计划项目(22YF7NA119;23YFNA0022);甘肃省高等学校产业支撑计划项目(2023CYZC-20);甘肃省科技重大专项(22ZD1NA001);甘肃省青年科技基金项目(24JRRA670);西北师范大学新农村发展研究院开放基金;西北师范大学研究生科研资助项目(2021KYZZ01037);西北师范大学优秀博士论文培养资助项目;甘肃省科技计划(优秀博士)资助项目(23JRRA697,23JRRA707)。

摘　　要：利用植物DORN1突变体(dorn1-3,其中T-DNA破坏了植物细胞外ATP受体DORN1编码基因),研究在盐胁迫下DORN1突变对拟南芥种子萌发及H_(2)O_(2)代谢的影响.结果表明,在盐胁迫下DORN1突变能够显著降低种子的萌发.盐胁迫显著提高了植物种子萌发中的H_(2)O_(2)含量和H_(2)O_(2)清除酶的活性;且H_(2)O_(2)的淬灭剂能够缓解盐胁迫对植物种子萌发的抑制,表明盐胁迫下种子萌发的下降和H_(2)O_(2)代谢密切相关.DORN1突变能够导致种子萌发中H_(2)O_(2)含量和H_(2)O_(2)清除酶活性的显著提高提示DORN1突变可能通过改变H_(2)O_(2)代谢影响盐胁迫下的种子萌发,表明在盐压力下细胞外ATP受体DORN1对拟南芥种子萌发具有重要的调节作用,且该作用与H_(2)O_(2)代谢有关.The effects of DORN1 mutation on the germination and H_(2)O_(2) metabolism of Arabidopsis thaliana seeds under salt stress were studied using DORN1 mutant(dorn1-3,in which T-DNA destroys the gene encoding exATP receptor DORN1).The results showed that DORN1 mutation could significantly reduce seed germination under salt stress,for the latter significantly increased the content of H_(2)O_(2) and the activities of H_(2)O_(2)-scavenging enzymes in seed germination.The quenching agent of H_(2)O_(2) could alleviate the inhibition of salt stress on the seed germination,indicating that a decrease of seed germination under salt stress could be closely related to H_(2)O_(2) metabolism.Further observation showed that under salt stress DORN1 mutation could significantly increase the content of H_(2)O_(2) and the activities of H_(2)O_(2)-scavenging enzymes during seed germination,suggesting that DORN1 mutation might affect seed germination under salt stress by altering the metabolism of H_(2)O_(2).These observations suggest that the exATP receptor DORN1 plays an important regulatory role in Arabidopsis seed germination under salt stress,and this role is related to H_(2)O_(2) metabolism.

关 键 词：盐胁迫 细胞外ATP受体 种子萌发 过氧化氢 

分 类 号：Q945[生物学—植物学]