基于网络药理、分子对接及实验验证探究藏药三果汤防治高脂血症的作用靶点及信号通路  

Based on Network Pharmacology,Molecular Docking and Experimental Verification,the Target and Signal Pathway of Sanguo Tang for Preventing and Treating Hyperlipidemia Were Explored

作　　者：卡毛加 夏吾扎西 先巴吉 才让南加 三智加[1] Kamao-Jia;Xiawu-Zhaxi;Xianba-ji;Cairang-Nanjia;Sanzhi-Jia(Qinghai University Tibetan Medical College,Xining 810016,China;Tibetan Hospital of Xizang Autonomous Region,Lhasa 850000,China;Huangnan Tibetan Hospital,Tongren 811399,China;Xizang Tibetan Medical University,Lhasa 850000,China)

机构地区：[1]青海大学藏医学院,青海西宁810016 [2]西藏自治区藏医院,西藏拉萨850000 [3]黄南藏族自治州藏医院,青海同仁811399 [4]西藏藏医药大学,西藏拉萨850000

出　　处：《高原科学研究》2024年第4期70-80,共11页Plateau Science Research

基　　金：国家重点研发计划项目(2017YFC1704001)。

摘　　要：目的:探讨藏药三果汤中潜在的治疗高血脂的活性成分及其作用靶点,初步阐明三果汤散降低血脂过程中的关键信号通路。方法:采用网络药理学、分子对接验证及通路富集分析,锁定三果汤降低血脂过程中的潜在效应分子MAPK,构建高血脂小鼠模型,并应用三果汤、MAPK抑制剂处理小鼠,Western blotting法检测小鼠体内蛋白分子及信号通路变化情况。结果:高血脂小鼠肝内PI3K/AKT信号通路磷酸化水平增加,并且上升幅度与小鼠血脂水平呈正相关。三果汤可通过抑制MAPK表达,降低PI3K/AKT信号通路磷酸化水平,从而发挥降血脂作用。结论:三果汤具有降血脂的功效,其作用机制与抑制MAPK表达、降低PI3K/AKT信号通路磷酸化水平有关。MAPK作为三果汤降血脂的作用靶点及信号通路值得进一步研究,而三果汤降低血脂的其他机制及效应分子也有待更深入的挖掘。Objective:To investigate the potential active components in San Guo Tang that treat hyperlipidemia and to elucidate the key signaling pathways involved in its lipid-lowering effects.Methods:We employed net-work pharmacology,molecular docking validation,and pathway enrichment analysis to identify the potential ef-fector molecule MAPK involved in the blood sugar-lowering process of San Guo Tang.A hyperlipidemia mouse model was established,and mice were treated with San Guo Tang and MAPK inhibitors.Western blotting was used to assess changes in protein molecules and signaling pathways within the mice.Results:The phosphoryla-tion level of PI3K/AKT signaling pathway in the liver of hyperlipidemic mice increased,and the magnitude of the increase was positively correlated with the blood lipid levels of the mice.San Guo Tang can inhibit the phosphory-lation of the PI3K/AKT signaling pathway by suppressing the expression of MAPK,thereby reducing blood lipid levels.Conclusion:San Guo Tang possesses lipid-lowering efficacy,which is associated with the inhibition of MAPK expression and the reduction of PI3K/AKT signaling pathway phosphorylation.Further research is war-ranted to explore MAPK as a target and signaling pathway for San Guo Tang’s lipid-lowering actions,as well as to uncover additional mechanisms and effector molecules involved in its lipid-lowering effects.

关 键 词：三果汤 高脂血症 治疗靶点 PI3K/AKT MAPK 

分 类 号：R589.2[医药卫生—内分泌]