不同连作年限西红花种植土壤微生物多样性及网络特征的差异  

Differences of Microbial Diversity and Network Characteristics in Crocus sativus-Planting Soil of Different Continuous Cropping Years

作　　者：李孟芝 封貌 高丽[1] 梁鹏 谢锐 申皓天 黄显章[1] LI Meng-zhi;FENG Mao;GAO Li(Henan Key Laboratory of Zhang Zhongjing Formulae and Herbs for Immunoregulation,Nanyang Institute of Technology,Nanyang,Henan 473000;Nanyang Institute of Technology,Nanyang,Henan 473000)

机构地区：[1]南阳理工学院河南省张仲景方药与免疫调节重点实验室,河南南阳473000 [2]南阳理工学院,河南南阳473000

出　　处：《安徽农业科学》2024年第24期137-144,176,共9页Journal of Anhui Agricultural Sciences

基　　金：河南省重大科技专项(221100310400);2022年度南阳理工学院交叉科学研究项目(520095);2022年度南阳市科学技术攻关项目(KJGG030)。

摘　　要：[目的]探究不同连作年限西红花种植土壤中细菌和真菌群落多样性以及网络结构特征。[方法]采用高通量测序技术分析不同连作年限西红花种植土壤微生物群落的多样性、组成以及网络特征差异。[结果]不同连作年限西红花种植土壤细菌和真菌的多样性发生明显变化,尤其是真菌的OTUs、Shannon、Simpson、Chao和ACE指数,随着连作年限增加呈现明显增加的趋势。主坐标分析(PCoA)和非度量多维尺度分析(NMDS)结果表明,不同连作年限西红花种植土壤中细菌和真菌群落组成均有明显差异。放线菌门(Actinobacteria)、变形菌门(Proteobacteria)、酸酐菌门(Acidobacteria)、绿弯菌门(Chloroflexi)和浮霉菌门(Planctomycetes)为4个不同连作年限西红花种植土壤中丰度前5位的细菌门。子囊菌门(Ascomycota)在4个连作年限中均为第一大真菌门(相对丰度>55%)。种植4年的土壤中放线菌门(Actinobacteria)和芽单胞菌门(Gemmatimonadetes)的丰度低于种植1年、2年和3年。产油菌属(Solicoccozyma)在连作4年西红花土壤中丰度显著高于其他年限(P<0.05)。细菌和真菌的共发生网络分析表明,不同连作年限西红花种植土壤细菌和真菌呈现不同的连接模式。[结论]该研究描绘了不同连作年限西红花种植土壤微生物多样性、组成以及网络结构的差异,从微生物角度探讨西红花连作障碍机制,为解决西红花的连作障碍提供参考。[Objective]To explore the diversity and network structure of bacterial and fungal communities in soil microorganism of Crocus sativus in different years of continuous cropping.[Method]High-throughput sequencing was carried out to determine the diversity,composition and network structure of bacterial and fungal communities in soil microorganism of C.sativus.[Result]The diversity of bacteria and fungi in soil of C.sativus had significant changes,especially the OTUs,Shannon,Simpson,Chao and ACE indices of fungi,which showed a significant increase trend with the increase of continuous cropping years.The results of PCoA and NMDS showed that there were significant differences in the composition of bacteria and fungi in soil of C.sativus in different years of continuous cropping.At the bacterial phylum level,Actinobacteria,Proteobacteria,Acidobacteria,Chloroflexi and Planctomycetes were the top five phyla.At the fungal phylum level,Ascomycota(55%)was the most abundant phylum.The relative abundance of Actinobacteria and Gemmatimonadetes were lower in soils of continuous cropping 4 year than continuous cropping 1,2 and 3 year.The relative abundance of Solicoccozyma was signifcantly higher in soils of continuous cropping 4 year than continuous cropping 1,2 and 3 year(P<0.05).There were different connectivity patterns of bacteria and fungi in C.sativus-planting soil of different continuous cropping years.[Conclusion]The study describes the differences of microbial diversity,composition and network structure in C.sativus-planting soil of different continuous cropping years,and explores the mechanism of C.sativus continuous cropping obstacles from a microbial perspective,providing reference for solving the obstacles of continuous cropping in C.sativus.

关 键 词：西红花 连作年限 土壤微生物 多样性 网络特征 

分 类 号：R282.2[医药卫生—中药学]