CD 163基因敲除猪对胸膜肺炎放线杆菌、猪链球菌和副猪嗜血杆菌的易感性研究  

Study on the Susceptibility of CD 163 Gene Knockout Pigs to Actinobacillus pleuropneumoniae,Streptococcus suis,and Haemophilus parasuis

作　　者：梁国濠 胡丹丹 钟海文 张健 杨德鸿 吴珍芳[1,2] 杨化强 张献伟[1,2] LIANG Guohao;HU Dandan;ZHONG Haiwen;ZHANG Jian;YANG Dehong;WU Zhenfang;YANG Huaqiang;ZHANG Xianwei(National Engineering Research Center For Swine Breeding Industry,College of Animal Science,South China Agricultural University,Guangzhou 510642,China;Wens Foodstuff Group Co.,Ltd.,Yunfu 527400,China)

机构地区：[1]华南农业大学动物科学学院/国家生猪种业工程技术中心,广州510642 [2]温氏食品集团股份有限公司,云浮527400

出　　处：《畜牧兽医学报》2024年第12期5478-5488,共11页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：科技创新2030-农业生物育种重大项目(2023ZD0404303-02)。

摘　　要：旨在探究在CD 163基因的SRCR5区域插入1个碱基的CD 163基因敲除(CD 163 gene knockout,CD 163-KO)型猪对3种关键细菌的易感性相较于野生型(wild-type,WT)猪是否会有所变化,评估CD 163-KO型猪的生物安全性。本研究选用均为50日龄、体重与健康状况等相似的17头CD 163-KO型大白猪和17头WT型大白猪,其中2头CD 163-KO型大白猪和2头WT型大白猪作为空白对照,不做任何感染处理。试验猪只均无胸膜肺炎放线杆菌(Actinobacillus pleuropneumoniae,APP)、猪链球菌(Streptococcus suis,SS)及副猪嗜血杆菌(Haemophlius parasuis,HPS)感染史与接种史。试验猪只随机分为3组,每组均有5头CD 163-KO型猪和WT型猪,A组接种APP1型菌株、B组接种SS2型菌株、C组接种HPS13型菌株。连续观察8 d,记录猪只临床症状表现与死亡情况;试验猪只死亡后及时解剖,观察心脏、胸腔、肺部等重要部位病变情况;采集肺样并提取肺部中的细菌DNA,用于定量PCR检测并统计分析与对比CD 163-KO型与WT型死亡大白猪肺中3种细菌分别的相对DNA拷贝量。依据试验猪只临床症状表现可得,各组试验猪只均成功感染相应的细菌,且各组内的CD 163-KO型猪与WT型猪的存活率均无显著差异(P>0.05);依据死亡猪只肺部病变情况及细菌定量PCR检测结果可得,CD 163-KO型死亡猪肺与WT型死亡猪肺中APP1型、SS2型及HPS13型菌株的相对DNA拷贝量均无显著差异(P>0.05)。本研究的结果是CD 163-KO型大白猪生物安全性的有力证据,证明CD 163基因敲除后没有提高猪只对APP、SS和HPS的易感性,不会显著影响猪只抵抗细菌的能力,为CD 163-KO型猪的生物安全性评估提供强有力的支持。This study aimed to investigate whether pigs with a CD 163 gene knockout(CD 163-KO)resulting from a single base insertion in the SRCR5 domain of the CD 163 gene exhibit altered susceptibility to 3 crucial bacterial pathogens compared to their wild-type(WT)counterparts and to evaluate the biosafety profile of CD 163-KO pigs.This study selected a total of 34 Large White pigs,17 CD 163-KO pigs and 17 wild-type pigs,all at the age of 50 days with similar body weights and health statuses.Among them,2 CD 163-KO Large White pigs and 2 WT Large White pigs were used as blank controls without any infection treatment.None of the pigs had a history of infection or vaccination against Actinobacillus pleuropneumoniae(APP),Streptococcus suis(SS),and Haemophilus parasuis(HPS).The pigs were randomly divided into 3 groups of 5 CD 163-KO and 5 WT pigs each:group A received APP serotype 1,group B received SS serotype 2,and group C received HPS serotype 13.The pigs were observed continuously for 8 days,the clinical symptoms and mortality were recorded.Upon death,the pigs were promptly necropsied to observe lesions in critical organs such as the heart,thorax,and lungs.Lung samples were collected,and bacterial DNA was extracted from the lungs for quantitative PCR analysis.The relative DNA copy numbers of the 3 bacterial species in the lungs of deceased CD 163-KO and WT pigs were statistically analyzed and compared.The primary aim of this study was to evaluate whether CD 163-KO pigs exhibit altered susceptibility to these 3 key bacterial pathogens compared to their WT counterparts,ultimately assessing the biosafety profile of CD 163-KO pigs.Based on the clinical manifestations observed in the experimental pigs,all groups successfully contracted the respective bacterial infections,and there were no significant differences in survival rates between CD 163-KO and WT pigs within each group(P>0.05).Furthermore,according to the pathological findings in the lungs of deceased pigs and the results of quantitative PCR analysis for bacterial DNA,n

关 键 词：CD 163 基因敲除 大白猪 猪繁殖与呼吸综合征病毒 胸膜肺炎放线杆菌 猪链球菌 副猪嗜血杆菌 生物安全评估 

分 类 号：S828.2[农业科学—畜牧学]