大鼠慢性内脏高敏性、肠道高表达TRPV1的模型构建  

Construction of Chronic Visceral Hypersensitivity andIntestinal Overexpression of TRPV1 in Rats

作　　者：梁倩莹 Liang Qianying(Guangzhou Women and Children’s Medical Center,Guangzhou Medical University,510515,China)

机构地区：[1]广州医科大学附属妇女儿童医疗中心,广州510515

出　　处：《黑龙江科学》2024年第24期128-130,134,共4页Heilongjiang Science

基　　金：国家自然科学基金项目资助(82003829);广州市科技计划项目资助(202102021103)。

摘　　要：建立慢性内脏高敏性(VHL)、肠道高表达TRPV1的大鼠模型,可为肠易激综合征患者靶向治疗药物的研发奠定动物实验基础。本实验采用辣椒素(Capsaicin, CAP)作刺激药物构建模型大鼠,应用腹部回撤反射(AWR)评分、RT-PCR和免疫印迹法(western blot)等技术评价不同浓度CAP大鼠肠道敏感性和TRPV1表达量。结果显示,0.1%~0.5%CAP连续给药后,实验组大鼠的AWR评分高于空白组大鼠。间隔刺激组与连续刺激组大鼠的AWR评分无显著差异,但HE染色结果表明,连续刺激组大鼠的肠黏膜组织损伤较多。RT-PCR和western blot检测发现,实验组大鼠注射0.1%~0.3%CAP的空肠、回肠、结肠均有一定程度的TRPV1表达增强作用。通过对不同浓度CAP和刺激间隔的条件分析,结合肠道组织HE染色和TRPV1表达量结果,最终选定0.3%CAP间隔1 d刺激14 d为构建慢性VHL兼TRPV1高表达的最佳大鼠模型。The establishment of a rat model of chronic visceral hypersensitivity(VHL)and intestinal high expression of TRPV1 can lay a foundation for the research and development of targeted therapeutic drugs for patients with IBS.The study constructs model rats with Capsaicin(CAP)as stimulant,evaluates the intestinal sensitivity and TRPV1 expression of rats with different concentrations of CAP through abdominal regression reflex(AWR)score,RT-PCR and western blot.The results show that after continuous administration of 0.1%~0.5%CAP,the AWR score of the experimental group is higher than that of the blank group.There is no significant difference in AWR scores between the interval stimulation group and the continuous stimulation group.But the results of HE staining show that there is no significant difference in AWR scores between the interval stimulation group and the continuous stimulation group.RT-PCR and western blot analysis show that the jejunum,ileum and colon of the experimental group of rats injected with 0.1%~0.3%CAP have enhanced TRPV1 expression to a certain extent.Through the condition analysis of different concentrations of CAP and stimulation intervals,and the combination with the results of intestinal tissue HE staining and TRPV1 expression,the study finally selects 0.3%CAP to intervel stimulation for 1 day and last 14 days as the best model of chronic VHL and high expression of TRPV1 in rats.

关 键 词：内脏高敏性 TRPV1 CAP 建模 

分 类 号：R714.2[医药卫生—妇产科学]