Deep tissue super-resolution imaging with adaptive optical two-photon multifocal structured illumination microscopy  

作　　者：Chenshuang Zhang Bin Yu Fangrui Lin Soham Samanta Huanhuan Yu Wei Zhang Yingying Jing Chunfeng Shang Danying Lin Ke Si Wei Gong Junle Qu 

机构地区：[1]Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province,College of Physics and Optoelectronic Engineering,Shenzhen University,Shenzhen 518060,China [2]Department of Computer Science and Technology,Anhui University of Finance and Economics,Bengbu 233030,China [3]Guangdong-Hongkong-Macao Institute of CNS Regeneration,Ministry of Education CNS Regeneration Collaborative Joint Laboratory,Jinan University,Guangzhou 510632,China [4]Shenzhen Institute of Neuroscience,Shenzhen 518057,China [5]MOE Frontier Science Center for Brain Science&Brain-Machine Integration,NHC and CAMS Key Laboratory of Medical Neurobiology,School of Brain Science and Brain Medicine,Zhejiang University,Hangzhou 310058,China

出　　处：《PhotoniX》2023年第1期1-15,共15页智汇光学（英文）

基　　金：National Key R&D Program of China(2021YFF0502900);National Natural Science Foundation of China(61975131,62175166,and 62127819);Shenzhen Key Laboratory of Photonics and Biophotonics(ZDSYS20210623092006020);Shenzhen Science and Technology Program(JCYJ20220818100202005,JCYJ20200109105411133).

摘　　要：Visualization of axons and dendritic spines is crucial in neuroscience research.However,traditional microscopy is limited by diffraction-limited resolution and shallow imaging depth,making it difficult to study neuronal dynamics.Two-photon multifocal structured illumination microscopy(2P-MSIM)provides super-resolution imaging along with a reasonably good penetration,but it is vulnerable to optical aberrations in deep tissues.Herein we present a novel non-inertial scanning 2P-MSIM system incorporated with adaptive optics(AO)which allows for super-resolution imaging with effective aberration correction.Our strategy is designed to correct both laser and fluorescence paths simultaneously using a spatial light modulator and a deformable mirror respectively,providing better results than the individual path corrections.The successful implementation of adaptive optical two-photon multifocal structured illumination microscopy(AO 2P-MSIM)has allowed for the super-resolution imaging of neuronal structures in a mouse brain slice at great depths and dynamic morphological characteristics of zebrafish motoneurons in vivo.

关 键 词：SUPER-RESOLUTION Adaptive optics In vivo imaging NEURONS 

分 类 号：O43[机械工程—光学工程]