小麦抗条锈基因Yr5、Yr9和Yr18分子标记的特异性评估  

Evaluation of Molecular Markers for Wheat Stripe Rust Resistance Genes Yr5,Yr9 and Yr18

作　　者：孙振宇[1] 黄亮 黄苗苗 刘太国[2] SUN Zhenyu;HUANG Liang;HUANG Miaomiao;LIU Taiguo(Institute of Plant Protection,Gansu Academy of Agricultural Sciences,Lanzhou,Gansu 730070 China;State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193 China;Academy of Agricultural and Forestry Sciences,Qinghai University,Xining,Qinghai 810016,China)

机构地区：[1]甘肃省农业科学院植物保护研究所,甘肃兰州730070 [2]中国农业科学院植物保护研究所,植物病虫害综合治理全国重点实验室,北京100193 [3]青海大学农林科学院,青海西宁810016

出　　处：《麦类作物学报》2025年第1期45-51,共7页Journal of Triticeae Crops

基　　金：国家自然科学基金项目(31660498);国家小麦产业技术体系项目(CARS-03);青海省自然科学基金项目(2022-ZJ-978Q)。

摘　　要：Yr5、Yr9(1B/1R)、Yr18基因的自身性状或连锁性状在中国小麦育种中具有重要的应用前景。本研究运用以Avocet S为背景的单基因近等基因系及其对应基因的载体品种,对Yr5基因的连锁标记STS9/10,Yr9(1B/1R)基因的分子标记AF1/4、D15、20H,Yr18基因的分子标记csLV34、cssfr1~cssfr5进行有效性验证。结果表明,Yr5基因的特异性分子标记STS9/10、Yr9(1B/1R)基因的特异性分子标记AF1/4、D15和20H均能够准确识别不同遗传背景材料中的相应抗性基因;Yr18基因分子标记cssfr2能够准确检测Yr18的非载体材料,并检测出Avocet S*6/Yr5、Avocet S*6/Yr24、Avocet S*6/Yr27三个材料具有Yr18的等位变异。这说明Yr5基因的连锁标记STS9/10,Yr9(1B/1R)基因的分子标记AF1/4、D15、20H能对目的基因进行有效检测,Yr18基因的几个分子标记结合使用不仅能够对目的基因进行有效检测,而且能够识别该位点的等位变异。The traits of Yr5,Yr9(1B/1R),and Yr18 genes play an important role in wheat breeding in China,and the molecular markers STS9/10 of Yr5,AF1/4,D15,20H of Yr9(1B/1R)and csLV34,cssfr1~cssfr5 of Yr18 were effectively tested by applying near isogenic lines under background of Avocet S and lines with known Yr genes.The results showed that the specific molecular markers of Yr5 gene STS9/10,Yr9(1B/1R)gene AF1/4,D15 and 20H could accurately identify the corresponding resistance genes in different genetic background materials.Yr18 gene molecular marker cssfr2 could accurately detect the materials not-carrying Yr18,and detect allelic variation of Yr18 in Avocet S*6/Yr5,Avocet S*6/Yr24,and Avocet S*6/Yr27.There fore,the linkage marker STS9/10 of Yr5 gene,the molecular markers AF1/4,D15 and 20H of Yr9(1B/1R)gene can effectively detect the target gene,and the combination of several molecular markers of Yr18 gene not only effectively detected the target gene but also identified the allelic variation of this site.

关 键 词：抗条锈基因 分子标记 特异性 

分 类 号：S512.1[农业科学—作物学] S330