Anti-inflammatory potential of black cumin seed oil and its nanoemulsion formulation against lipopolysaccharide-induced liver injury in mice  

作　　者：Hesham Haffez Rehab Hosni Shady A.Swidan Hatem K.Amin 

机构地区：[1]Biochemistry and Molecular Biology Department,Faculty of Pharmacy,Helwan University,Helwan,11795,Cairo,Egypt [2]Center of Scientific Excellence"Helwan Structural Biology Research",Helwan University,Helwan,11795,Cairo,Egypt [3]El Sisi Comprehensive Clinic,General Authority for Health Insurance-Giza,Haram,12556,Giza,Egypt [4]Department of Pharmaceutics and Pharmaceutical Technology,Faculty of Pharmacy,The British University in Egypt,El-Sherouk,11837,Cairo,Egypt [5]Biochemistry Department,Faculty of Pharmacy,Galala University,Attaka,43511,Suez,Egypt

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2024年第12期523-531,共9页亚太热带生物医学杂志（英文版）

摘　　要：Objective:To evaluate the anti-inflammatory effect of black cumin seed oil(BCSO)and its nanoemulsion on lipopolysaccharide(LPS)-induced liver injury in mice.Methods:LPS-induced acute liver injury mouse model was used to evaluate the effects of BCSO and its nanoemulsion formulation on liver function.Hepatic inflammatory markers including Toll-like receptor 4(TLR4),interleukin(IL)-1β,heme-oxygenase 1,BAX,and BCL-2 were assessed using real-time PCR.Additionally,protein levels of reduced glutathione,tumor necrosis factor-α,and IL-6 were measured using ELISA,and histological analysis was performed.Indomethacin was used as a standard positive control for comparison.Results:BCSO reduced LPS-induced liver injury and exhibited strong anti-inflammatory effects by downregulating the expression of TLR4,IL-1β,IL-6,tumor necrosis factor-α,and heme-oxygenase 1.Additionally,BCSO demonstrated antioxidant properties by increasing reduced glutathione protein levels and decreasing key apoptotic markers BAX and BCL-2 in hepatocytes.The nanoemulsion formulation further enhanced these anti-inflammatory,antioxidant,and anti-apoptotic effects,and histological examination confirmed this effect.Combining BCSO with indomethacin at a lower dose improved efficacy,thereby reducing its potential side effects.Conclusions:The investigation reveals the anti-inflammatory impact of BCSO and its nanoemulsion formulation on LPS-induced liver oxidative stress,inflammation,and apoptosis.

关 键 词：Black cumin seed oil Liver inflammation LIPOPOLYSACCHARIDE Oxidative stress IL-6 TLR4 Apoptosis 

分 类 号：R965[医药卫生—药理学]