注射用红花黄色素对阿司匹林的药动学和抗凝作用的影响研究  

Effect of safflower yellow injection on the pharmacokinetics and anticoagulation of aspirin

作　　者：沈丹丹[1] 杨忠慧[1] 黄吉 偶鹰飞[1] 单云龙 SHEN Dandan;YANG Zhonghui;HUANG Ji;OU Yingfei;SHAN Yunlong(Department of Pharmacy,The First People’s Hospital of Taicang,Affiliated Hospital of Soochow University,Taicang 215400;Key Laboratory of Drug Metabolism and Pharmacokinetics,China Pharmaceutical University,Nanjing 210009,China)

机构地区：[1]太仓市第一人民医院,苏州大学附属太仓医院,太仓215400 [2]中国药科大学药物代谢动力学重点实验室,南京210009

出　　处：《中国药科大学学报》2024年第6期795-800,共6页Journal of China Pharmaceutical University

基　　金：太仓市科技计划项目(TC2020JCYL04)。

摘　　要：从药动学角度出发结合抗凝等药效学指标,探讨注射用红花黄色素(safflower yellow injection,SYI)对阿司匹林作用的影响。采用定量高效液相色谱(HPLC)分析法检测血浆样品中水杨酸,评价阿司匹林与SYI联合使用后对阿司匹林的水解产物水杨酸的药动学影响;采用试剂盒检测血浆血栓烷B2(thromboxane B2,TXB2)和6-酮-前列腺素F1α(6-keto-PGF1α),天冬氨酸转氨酶(aspartate aminotranferase,AST)、丙氨酸转氨酶(alanine aminotransferase,ALT)、血清尿素氮(blood urea nitrogen,BUN)、血肌酐(serum creatinine,Scr)水平,评价2种药物长期联合使用后抗凝效果及安全性指标。结果显示,与阿司匹林组比较,联合使用红花黄色素组的大鼠血浆药动学参数无显著差异(P>0.05);此外,联合使用组的血浆TXB2与阿司匹林组相比显著下降(P<0.01)。但6-keto-PGF1α水平无显著差异(P>0.05);治疗前后两组血清BUN、Scr、AST和ALT水平比较,差异均无统计学意义(P>0.05)。研究结果表明,SYI与阿司匹林联用不会引起水杨酸血药浓度明显改变,对阿司匹林的抗血小板作用没有影响。This article explores the interaction between aspirin and safflower yellow injection from the perspective of pharmacokinetics,combined with pharmacological indicators such as anticoagulation.Quantitative HPLC analysis was performed for the detection of salicylic acid in plasma samples,and the pharmacokinetic effects of aspirin in combination with safflower yellow injection on aspirin’s hydrolyzed product salicylic acid was evaluated.Thromboxane B2(TXB2)and 6-keto prostaglandin F1 in plasma were determined using enzymelinked immunosorbent assay and fully automated biochemical analyzerα(6-keto-PGF1)α,and the levels of aspartate aminotransferase(AST),alanine aminotransferase(ALT),blood urea nitrogen(BUN),and serum creatinine(Scr)were tested to evaluate the anticoagulant effect and safety indicators after long-term combined use of the two drugs,including liver and kidney function and cardiac pathological examination.The results showed that,compared with the aspirin group,there was no significant difference(P>0.05)in the plasma pharmacokinetic parameters of rats with combined use of safflower yellow injection.In addition,the plasma TXB2 in the combination group was significantly reduced compared to the aspirin group(P<0.01),yet with no significant difference for 6-keto-PGF1α(P>0.05).There was no statistically significant difference in the levels of serum BUN,Scr,AST,and ALT between the two groups before and after treatment(P>0.05).These results suggest that the combination of aspirin and safflower yellow injection does not cause significant change in the blood concentration of salicylic acid.It does not affect the antiplatelet effect of aspirin.

关 键 词：阿司匹林 红花黄色素 药物相互作用 水杨酸 

分 类 号：R969[医药卫生—药理学]