Geographic variation in secondary metabolites contents and their relationship with soil mineral elements in Pleuropterus multiflorum Thunb.from different regions  

作　　者：Yaling Yang Siman Wang Ruibin Bai Feng Xiong Yan Jin Hanwei Liu ZiyiWang Chengyuan Yang Yi Yu Apu Chowdhury Chuanzhi Kang Jian Yang Lanping Guo 

机构地区：[1]State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing,China [2]Key Laboratory of Biology and Cultivation of Herb Medicine,Ministry of Agriculture and Rural Affairs,Beijing,China [3]Ningbo Customs District Technology Center,Ningbo,China [4]Ningbo Joysun Product Testing Service Co.,Ltd,Ningbo,China [5]Infinitus(China)Company Ltd,Guangzhou,China [6]Keenan Research Center for Biomedical Science,St.Micheal's Hospital,Toronto,Ontario,Canada

出　　处：《Science of Traditional Chinese Medicine》2024年第3期214-223,共10页中医药科学（英文）

基　　金：financially supported by the scientific and technological innovation project of the China Academy of Chinese Medical Sciences(Grant Nos.CI2021A03905,CI2021A04011,CI2021A04005);Quality and Technical Service Platform for Traditional Chinese Medicine Whole Industry Chain(Grant No.2022-230-221);China Agricultural Research SystemofMOF andMARA(CARS-21);the Key Project at Central Government Level:the ability establishment of sustainable use for valuable Chinese medicine resources(Grant No.2060302).

摘　　要：Background:Pleuropterus multiflorum Thunb.cv.“Heshouwu”(HSW)has been used as a classical material for both medicine and food in China for millennia.Recently,the cultivation region of HSW has shifted from Guangdong to Sichuan,Guizhou,and other regions.The investigation of geographic variation in bioactive metabolite contents and their relationship with soil mineral elements holds academic significance.Objective:This study aimed to investigate the variations in the distribution of active components in HSWacross diverse planting regions and their relationship with soil mineral elements.Methods:A reliable quantitative analysis based on ultrahigh-performance liquid chromatography with triple-quadrupole mass spectrometry(UPLC-QQQ-MS)was developed to assess the levels of 15 bioactivemetabolites in 60 HSWsamples collected from 4 distinct regions.A total of 43 soil mineral elements in corresponding 60 soil samples were quantified by inductively coupled plasma mass spectrometry(ICP-MS).Orthogonal partial least squares-discriminant analysis(OPLS-DA),heatmap analysis,Pearson correlation analysis,and random forest(RF)regression were conducted based on the above quantitative data.Results:The content of stilbene glycosides displayed a wider range of variation compared with emodin and physcion among different regions.Eight compounds were screened as the differential metabolites in HSW samples from various sources using the supervised OPLS-DA analysis.Among these,2 important functional compounds including physcion and 2,3,5,4′-tetrahydroxystilbene-2-O-(6″-O-acetyl)-glucoside(THSG-5)are the most abundant inHSWsamples from Deqing,a geographical indicative production region.Pearson correlation analysis indicated that the impact of soil mineral elements on the levels of stilbene glycosides is greater compared to that on anthraquinones.A negative correlation was observed between the levels of elements Na,Zn,Ba,Ti,and 2,3,5,4′-tetrahydroxysilbene 2-O-glucoside(THSG-1).Conversely,a positive correlation was found between the co

关 键 词：Pleuropterus multiflorum Thunb. Geographical difference Soil mineral element UPLC-QQQ-MS Stilbene glycosides ANTHRAQUINONES 

分 类 号：O62[理学—有机化学]