Improvement of myocardial fibrosis injury by Shengmai injection in ischemia-induced heart failure in a rat model  

作　　者：Yurong Ding Junying Wei Enhui Ji Menglan Wang Yue Wu Mengli Chang Yi Zhang Jing Xu 

机构地区：[1]Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing,China [2]Civil Aviation General Hospital,Civil Aviation Medical Center,Civil Aviation Administration of China,Beijing,China

出　　处：《Science of Traditional Chinese Medicine》2024年第3期245-253,共9页中医药科学（英文）

基　　金：supported by the national key research and development program of China(2019YFC1708900);National Natural Science Foundation of China(82204973);scientific and technological innovation project of China Academy of Chinese Medical Sciences(CI2021B015);the fundamental research funds for the central public welfare research institutes of Institute of Chinese Materia Medica,China Academy ofChinese Medical Sciences(ZXKT21030);special program for outstanding,young scientific and technological talents(innovation)of China Academy of ChineseMedical Sciences(ZZ15-YQ-034).

摘　　要：Background:Heart failure(HF)is the leading cause of death worldwide.Myocardial infarction(MI)is a major contributor to HF.Shengmai injection(SMI)has exhibited protective efficacy in preventing HF.However,the advantages of SMI in the progression of MI-induced HF remain unclear.Objective:To reveal the advantages of SMI in the progression of MI-induced HF.Methods:The differently expressed proteins in rat models with ischemia at the 7th,14th,21st,and 28th days were obtained from PubMed.The“compound-target”network of SMI was constructed via the Bioinformatics Analysis Tool for Molecular mechANism of Traditional Chinese Medicine database.The protein-protein interaction relationship was constructed,and biological function was applied to evaluate the advantage effect of SMI in the progression of MI-induced HF.In addition,the prediction results were validated in rats with left anterior descending coronary artery ligation.The cardiac function and heart performance were observed via echocardiography,hematoxylin-eosin staining,and Masson staining,and the levels of procollagen type I carboxy-terminal propeptide,recombinant versican(VCAN),and collagen 1A1(COL1A1)weremeasured via enzyme-linked immunosorbent assay in rat plasma.In vitro,H9c2 cells were treated with Angiotensin II(Ang II),and the cell viability,the level of reactive oxygen species(ROS)and Ca^(2+),and the expression of ANP and connective tissue growth factor were evaluated.Furthermore,the schizandrin A was identified as one of the possible key compounds.After schizandrin A treatment,the level of ROS and Ca^(2+)and the expression of COL1A1 and VCAN were evaluated.Results:There were 189 compounds and 1612 targets involved in the“compound-target”network,and an interaction relationship was constructed.According to the top subnetwork,the Gene Ontology annotation revealed that SMI may have an antifibrotic and cardiac protective effect against MI-induced HF.In rats,SMI increased ejection fraction,left ventricular fractional shortening,and cardiac output and decre

关 键 词：Shengmai injection Proteomic profile Heart failure Myocardial fibrosis Myocardial infarction 

分 类 号：R541.6[医药卫生—心血管疾病]