白枇杷微生物组及其与果实品质的关系  

Relationships between microbiome and fruit quality of white loquat

作　　者：赵宇卓 吴碧波 余朝旭 钟莺莺 樊树雷 陈丹 肖谋良 王丽[8] 葛体达 吴愉萍 ZHAO Yuzhuo;WU Bibo;YU Zhaoxu;ZHONG Yingying;FAN Shulei;CHEN Dan;XIAO Mouliang;WANG Li;GE Tida;WU Yuping(Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment,College of Environmental and Resource Sciences,Zhejiang University,Hangzhou 310058,Zhejiang,China;Ninghai County Forestry Specialty Technology Promotion Station,Ningbo 315600,Zhejiang,China;Xiangshan County Agriculture and Rural Green Development Center,Ningbo 315700,Zhejiang,China;Ningbo Customs Technical Center,Ningbo 315000,Zhejiang,China;Ningbo Agricultural Technology Extension Station,Ningbo 315012,Zhejiang,China;Ninghai County Agricultural Product Quality and Safety Management Station,Ningbo 315600,Zhejiang,China;College of Environment and Resources,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China;State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products,Institute of Plant Virology,Ningbo University,Ningbo 315211,Zhejiang,China;Ningbo Agriculture and Rural Green Development Center,Ningbo 315012,Zhejiang,China)

机构地区：[1]浙江大学环境与资源学院,浙江省农业资源与环境重点实验室,浙江杭州310058 [2]宁海县林特技术推广站,浙江宁波315600 [3]象山县农业农村绿色发展中心,浙江宁波315700 [4]宁波海关技术中心,浙江宁波315000 [5]宁波市农业技术推广总站,浙江宁波315012 [6]宁海县农产品质量安全管理站,浙江宁波315600 [7]浙江农林大学环境与资源学院,浙江杭州311300 [8]宁波大学植物病毒学研究所,农产品质量安全危害因子与风险防控国家重点实验室,浙江宁波315211 [9]宁波市农业农村绿色发展中心,浙江宁波315012

出　　处：《浙江大学学报（农业与生命科学版）》2024年第6期907-919,共13页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：浙江省宁波市公益类重点科技项目(2021S013);浙江省宁波市象山县科技计划项目“象山枇杷精准施肥增效关键技术研究与示范”(2022C1018)。

摘　　要：植物微生物组是植物健康和生产力的关键决定因素之一,为宿主植物提供如获取低丰度营养物质、抵抗病原体入侵及适应环境等功能。本研究以白枇杷为研究对象,全面地测定了果实的风味物质和理化指标,并基于16S rRNA扩增子技术分析白枇杷根内、叶内及根周土壤环境中微生物的组成及功能,揭示白枇杷微生物组与果实品质的关系。结果表明:土壤环境中的微生物群落涵盖大部分根内、叶内环境中的微生物,但它们在群落结构和功能上具有明显的差异,说明植物不同部位的内生微生物存在明显的生态位分化现象;通过分析核心菌群与非核心菌群,发现在3种环境中均存在功能冗余现象;基于果实品质与微生物的相关性分析,发现根内微生物对果实品质有明显的调控作用,而在叶内没有发现。本研究明确了白枇杷中微生物-宿主的互作关系,并找出了与果实品质相关联的微生物,为白枇杷精准施肥增效奠定了重要的研究基础。The plant microbiome is one of the key determinants of plant health and productivity,providing host plants with functions such as access to low-abundance nutrients,resistance to pathogen invasion,and adaptation to the environment.In this study,the flavor and physicochemical indexes of white loquat were comprehensively determined,and 16S rRNA amplicon technology was used to analyze the composition and function of microorganisms in the roots,leaves and bulk soil environments of white loquat,in order to reveal the relationships between the microbial community and fruit quality.The results showed that the microbial communities in soil environment covered most of the microorganisms in the root and leaf environments,but there were obvious differences in community structure and microbial function,indicating that there was obvious niche differentiation of endophytic microorganisms in different organs of plants.An analysis of the core microbiome and non-core microbiome revealed that functional redundancy existed in all three environments.On the basis of the correlation analysis between fruit quality and microorganisms,it was found that microorganisms in roots had an obvious regulatory effect on fruit quality but were not found in leaves.This study clarifies the microbiome-host interaction in white loquat and identifies the microorganisms associated with fruit quality,laying an important foundation for the study of precision fertilization and efficiency enhancement in white loquat.

关 键 词：植物微生物组 白枇杷 果实品质 扩增子测序 生态位分化 

分 类 号：S667.3[农业科学—果树学]