SD大鼠急性高原缺氧缺血性脑损伤模型的建立与评价  

Establishment and Evaluation of an Acute Plateau Hypoxic-ischemic Brain Injury Model in SD Rats

作　　者：马博华 李萌 李倩 邓成程 王瑞[1] 尹东锋[1] MA Bo-hua;LI Meng;LI Qian;DENG Cheng-cheng;WANG Rui;YIN Dong-feng(Xinjiang Military General Hospital of PLA,Urumqi 830000,China;Qingyang People's Hospital,Qingyang 745000,China)

机构地区：[1]新疆军区总医院,新疆乌鲁木齐830000 [2]庆阳市人民医院,甘肃庆阳745000

出　　处：《解放军药学学报》2024年第6期507-510,共4页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：新疆维吾尔自治区重点研发计划项目子课题-2,No.2022B03005-2。

摘　　要：目的建立SD大鼠高原缺氧缺血性脑损伤模型。方法60只SD大鼠随机分为常氧对照组和缺氧缺血模型组,常氧对照组于平原条件下(海拔约800m)喂养,缺氧缺血模型组结扎右颈总动脉2h后置于低压氧舱内,模拟海拔6000m高原环境,分为缺氧缺血1、3、5、7d组,每组12只;缺氧缺血暴露结束后,出舱,称量并记录大鼠体质量及脑组织干湿质量,计算脑含水量;ELISA法测定脑海马组织中血管内皮生长因子、肿瘤坏死因子、白介素1β(IL-1β)和白介素6(IL-6);HE染色观察脑组织病理变化。结果与常氧对照组比较,缺氧缺血组1、3和5d组脑含水量升高显著;缺氧缺血7d组无显著差异;缺氧缺血1、3、5d组中的IL-1β、IL-6、血管内皮生长因子和肿瘤坏死因子均显著升高;而缺氧缺血7d组肿瘤坏死因子无显著变化,IL-1β、IL-6和血管内皮生长因子有显著升高;镜下观察常氧对照组脑组织结构完整,神经元细胞形态正常,海马细胞排列整齐,血管外周间隙小,缺氧缺血3和5d组大鼠脑组织神经细胞减少显著,部分细胞出现空泡,分子层、椎体层细胞水肿明显,血管周间隙增大显著;缺氧缺血1d组有少部分细胞分子层、锥体层水肿,神经细胞排列较整齐;缺氧缺血7d组无显著变化。结论建立了高原缺氧缺血性脑损伤大鼠模型,并发现缺氧缺血3d组动物模型更为稳定。Objective To establish a plateau model of hypoxic-ischemic brain injury in SD rats.Methods Sixty SD rats were randomly divided into the normoxic control group and hypoxic-ischemic model group.The normoxic control group was fed on simulated plains(about 800 m),while the hypoxic-ischemic model group was placed in a low-pressure oxygen chamber,which simulated a 6000 m plateau environment,two hours after ligating the right common carotid artery.After exposures to hypoxia and ischemia,the rats were weighed,the body weight and wet and dry weight of brain tissue were recorded,and the brain water content was calculated.ELISA was used to determine the vascular endothelial growth factor(VEGF),tumor necrosis factor(TNF-α),interleukin 1β(IL-1β)and interleukin 6(IL-6)in brain tissue.HE staining was used to observe the pathological changes of brain tissue.Results Compared with the normoxic control group,there was a significant increase in brain water contents in the hypoxic-ischemic group at 1,3 and 5 d,but there was no significant change in the hypoxic-ischemic group at 7 d.IL-1β,IL-6,VEGF and TNF-αwere significantly increased in the hypoxic-ischemic group at 1,3 and 5 d(P<0.01).There was no significant change in TNF-αin the 7 d hypoxic-ischemic group,but IL-1β,IL-6 and VEGF were significantly increased in the 7 d hypoxic-ischemic group.Microscopically,the brain tissue of the normoxic control group was structurally intact,with normal neuronal cell morphology,neatly arranged cells in the hippocampal region and small peripheral gaps in the blood vessels.After treatment,the neuronal cells in the brain tissue of rats in the 3 and 5 d groups decreased obviously,some cells appeared vacuolated,the cells in the molecular layer and vertebral body layer were edematous,and the perivascular gap increased obviously.A few cells in the 1 d group had edema in the molecular layer and cone layer,and the neuronal cells were arranged neatly,but there was no significantly change in the 7 d group.Conclusion A rat model of high-altitude hypo

关 键 词：缺氧 缺血 脑损伤 大鼠 

分 类 号：R965[医药卫生—药理学]