线性钆沉积大鼠模型使用不同类型钆剂后腹部器官钆沉积的变化  

Changes of the Gadolinium Deposition in Abdominal Organs After Administration of Different Types of Gadolinium Agents in Linear Gadolinium Deposition Model in Rats

作　　者：王旭聪 李健 陈兵[1] WANG Xucong;LI Jian;CHEN Bing(Department of Radiology,General Hospital of Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]宁夏医科大学总医院放射科,宁夏银川750004

出　　处：《中国医学影像学杂志》2024年第12期1203-1208,共6页Chinese Journal of Medical Imaging

基　　金：中国红十字基金会“影瑞西北公益行”之ICON科研基金项目(XM_HR_ICON_2020_10)。

摘　　要：目的 探究线性钆剂沉积模型大鼠注射不同类型钆剂后腹部器官内钆沉积的变化。材料与方法 使用28只雄性SD大鼠建立初步线性钆沉积大鼠模型(单次剂量为0.06 mmol/kg,4次/周,共5周,注射线性钆剂钆双胺20次)。再随机分成4组分别注射钆双胺(线性),钆特酸葡胺(大环型),钆布醇(大环型)及高渗盐水,注射方式及周期同前。在第5、10周分批安乐死大鼠后提取肾脏、肝脏和脾脏组织,通过电感耦合等离子体质谱进行组织内钆浓度测定,使用透射电子显微镜及X射线光谱仪观察分析组织内钆微粒分布情况,并进行HE染色进行病理学观察。结果 各组内钆浓度(肝脏、脾脏、肾脏)及各组间钆浓度(线性组、大环组、第5周处死组、高渗盐水组)差异均有统计学意义(H=12.82~21.10,P均<0.01)。其中,线性组钆沉积浓度远高于其他组(P<0.01),大环组次之,高渗盐水组最低;各组内肾脏钆浓度远高于肝脏及脾脏(P<0.01)。透射电子显微镜和X射线光谱仪证实组织中钆微粒沉积,以线性组最显著。HE染色示各组均有肾小球硬化及肾小管弥漫性扩张改变,以线性组显著;各组内肝脏和脾脏未见明显组织学损伤及差异。结论 在线性钆沉积模型大鼠中使用大环型钆对比剂后腹部器官钆沉积较少,且对肾脏组织损伤影响较小。本研究为临床中既往已使用线性钆剂的人群再次选用钆剂提供重要参考依据。Purpose To investigate the changes of gadolinium deposition in abdominal organs after continuous injection of different types of gadolinium agents of a linear gadolinium deposition model in rats.Materials and Methods A preliminary model of linear gadolinium deposition was established in 28 male SD rats.Firstly,they were first injected with gadodiamide(linear gadolinium)20 times(A single dose of 0.06 mmol/kg was given;4 times per week for 5 weeks).Randomly,they were divided into four groups:Omniscan(linear),gadolinium DOTA meglumine(macrocyclic),gadobutrol(macrocyclic)and hypertonic saline.The number and dose of injections were the same as before.At the 5th and 10th week after euthanasia,the kidney,liver and spleen tissues were extracted for inductively coupled plasma mass spectrometry to determine the concentration of gadolinium in the tissues,and the distribution of gadolinium particles in the tissues was observed and analyzed by transmission electron microscopy and X-ray energy spectrometer,and HE staining was performed for pathological observation.Results The gadolinium concentration in liver and spleen and kidney of all groups and the gadolinium concentration among all groups were statistically significant(H=12.82-21.10,P<0.01).The gadolinium deposition concentration in gadolinium diamine group was significantly higher than that in other groups(P<0.01),followed by macrocyclic group,and the lowest in hypertonic saline group.The concentration of gadolinium in kidney was significantly higher than that in liver and spleen(P<0.01).Transmission electron microscopy and X-ray energy spectrometer confirmed the deposition of gadolinium particles in tissues,and which were the most significant in gadodiamide group.HE staining showed that there were glomerular sclerosis and diffuse dilatation of renal tubules in all groups,which were significant in gadodiamide group.There was no significant histological injury or difference in liver and spleen among all groups.Conclusion In rats with linear gadolinium deposition,macrocycl

关 键 词：钆 造影剂 腹部 质谱法 大鼠 病理学 

分 类 号：R814[医药卫生—影像医学与核医学] R981[医药卫生—放射医学]