血小板衍生生长因子DD对人肌腱源性干细胞增殖和多向分化能力的影响  

Effects of platelet-derived growth factor-DD on proliferation and multilineage differentiation of human tendon-derived stem cells

作　　者：文华伟 张青松 汤明 李亚楠 谈鸿飞 方禹舜 Wen Huawei;Zhang Qingsong;Tang Ming;Li Yanan;Tan Hongfei;Fang Yushun(Wuhan Fourth Hospital,Wuhan 430030,Hubei Province,China;Hubei Sports Medicine Center,Wuhan 430030,Hubei Province,China)

机构地区：[1]武汉市第四医院,湖北省武汉市430030 [2]湖北省运动医学中心,湖北省武汉市430030

出　　处：《中国组织工程研究》2025年第31期6649-6655,共7页Chinese Journal of Tissue Engineering Research

基　　金：湖北省自然科学基金(2022CFC054),项目负责人:方禹舜;湖北省自然科学基金(2021CFB520),项目负责人:张青松;湖北省自然科学基金(2022CFB517),项目负责人:汤明;武汉市卫健委重大项目(WX20B12),项目负责人:方禹舜。

摘　　要：背景:慢性肩袖损伤常伴有肌腱退变和肌腱干细胞功能受损,血小板衍生生长因子DD作为一种重要的细胞因子,对干细胞增殖和分化具有调节作用。目的:探讨血小板衍生生长因子DD对人慢性肩袖损伤组织来源肌腱干细胞增殖和多向分化能力的影响。方法:获取人体慢性肩袖损伤组织标本并体外分离培养肌腱干细胞,免疫荧光染色观察细胞的骨架形态,流式检测肌腱干细胞表型表达。将肌腱干细胞分成2组:对照组不进行任何干预,血小板衍生生长因子DD组以5μg/mL血小板衍生生长因子DD干预,通过细胞增殖实验和三系分化实验评估血小板衍生生长因子DD对肌腱干细胞增殖和多向分化能力的影响。结果与结论:①血小板衍生生长因子DD组EdU阳性细胞数量较对照组显著增加(P<0.05),且肌腱干细胞更早进入快速增殖期,细胞呈对数增长;②血小板衍生生长因子DD组油红O染色、阿利新蓝染色、茜素红染色阳性面积较对照组明显增大(P<0.05);③上述结果表明,血小板衍生生长因子DD显著促进肌腱干细胞的增殖和成脂、成骨、成软骨分化。BACKGROUND:Chronic rotator cuff injury is often companied by tendon degeneration and impaired function of tendon-derived stem cells.As am important cytokine,platelet-derived growth factor-DD has a regulatory effect on the proliferation and differentiation of tendon-derived stem cells.OBJECTIVE:To investigate the effect of platelet-derived growth factor-DD on the proliferation and multilineage differentiation of tendon-derived stem cells in human chronic rotator cuff injury.METHODS:Tendon-derived stem cells were isolated from human chronic rotator cuff injury tissue and cultured in vitro.Immunofluorescence staining was used to observe the cytoskeletal morphology of tendon-derived stem cells.Flow cytometry was used to identify the phenotype of tendon-derived stem cells.Tendon-derived stem cells were divided into two groups.The control group did not receive any intervention.The platelet-derived growth factor-DD group was treated with 5μg/mL platelet-derived growth factor-DD.The effect of platelet-derived growth factor-DD on the proliferation and multilineage differentiation of tendon-derived stem cells was evaluated by cell proliferation assay and three-lineage differentiation assay.RESULTS AND CONCLUSION:(1)The number of EdU-positive cells in the platelet-derived growth factor-DD group was significantly increased compared with the control group(P<0.05).Tendon-derived stem cells entered the rapid proliferation phase earlier,and the cell growth was logarithmic.(2)The positive areas of Oil Red O staining,Alcian Blue staining,and Alizarin Red staining in the platelet-derived growth factor-DD group were significantly larger than those in the control group(P<0.05).(3)The above results show that platelet-derived growth factor-DD significantly promotes the proliferation and adipogenic,osteogenic,and chondrogenic differentiation of tendon-derived stem cells.

关 键 词：肌腱干细胞 肌腱源性干细胞 血小板衍生生长因子DD 肩袖损伤 肩袖修复 工程化干细胞 

分 类 号：R459.9[医药卫生—治疗学] R318.5[医药卫生—临床医学] R686.1