白细胞介素18在低氧暴露大鼠骨髓及外周血中表达的意义  

Significance of interleukin-18 expression in bone marrow and peripheral blood of rats exposed to hypoxia

作　　者：李金杰 肖经雪 李楠 宋珍 周彦云 马婕[1] Li Jinjie;Xiao Jingxue;Li Nan;Song Zhen;Zhou Yanyun;Ma Jie(Qinghai University Affiliated Hospital(Clinical Medicine School),Xining 810001,Qinghai Province,China)

机构地区：[1]青海大学附属医院(临床医学院),青海省西宁市810001

出　　处：《中国组织工程研究》2025年第31期6681-6687,共7页Chinese Journal of Tissue Engineering Research

基　　金：青海省“昆仑英才·高端创新创业人才”计划(青才人字2020-18号),项目负责人:马婕;青海省科技厅自然科学基金项目(2020-ZJ-956Q),项目负责人:马婕。

摘　　要：背景:低氧暴露下大鼠外周血红细胞水平明显升高,而骨髓有核红细胞增殖可能是导致外周血红细胞增加的直接原因之一,既往研究着重于促红细胞生成素、低氧诱导因子等因素的影响,对炎症、免疫等相关因素的研究甚少。目的:研究低氧暴露后大鼠骨髓有核红细胞、骨髓上清液及外周血中白细胞介素18的表达,探讨白细胞介素18在慢性高原病发病过程中可能发挥的作用。方法:16只健康雄性SD大鼠随机分为2组:实验组在模拟海拔5000 m低压氧舱中饲养28 d,对照组在海拔2260 m的实验室内饲养28 d,检测两组大鼠血常规,流式测定两组大鼠骨髓CD71^(+)有核红细胞比例,RT-qPCR、Western blot测定两组大鼠骨髓CD71^(+)有核红细胞中白细胞介素18 mRNA和蛋白表达,免疫荧光测定两组大鼠胸骨中白细胞介素18蛋白表达,ELISA测定两组大鼠外周血、骨髓上清液中白细胞介素18水平。结果与结论:①实验组大鼠外周血红细胞计数、血红蛋白、红细胞压积、平均血红蛋白含量等指标均较对照组增高(P<0.05);②实验组大鼠骨髓CD71^(+)有核红细胞比例较对照组显著增高(P<0.05);③RT-qPCR结果显示,实验组大鼠骨髓CD71^(+)有核红细胞中白细胞介素18 mRNA表达较对照组显著增高(P<0.05);④Western blot结果显示,实验组大鼠骨髓CD71^(+)有核红细胞中白细胞介素18蛋白表达较对照组显著增高(P<0.05);⑤免疫荧光结果显示,实验组大鼠胸骨中白细胞介素18蛋白表达较对照组显著增高(P<0.05);⑥ELISA结果显示,实验组大鼠血清中白细胞介素18水平较对照组增高(P<0.05),但实验组大鼠骨髓上清液中白细胞介素18水平较对照组下降(P<0.05)。结果表明低氧暴露后大鼠骨髓CD71^(+)有核红细胞、外周血中白细胞介素18表达增高可能参与骨髓有核红细胞的增殖过程。BACKGROUND:The level of peripheral erythrocytes in rats is significantly increased under hypoxia exposure,and the proliferation of nucleated erythrocytes in the bone marrow may be one of the direct causes of the increase in peripheral erythrocytes.Previous studies have focused on the effects of factors such as erythropoietin and hypoxia-inducible factor,but little research has been done on related factors such as inflammation and immunity.OBJECTIVE:To study the expression of interleukin-18 in bone marrow nucleated erythrocytes,bone marrow supernatant and peripheral blood of rats after hypoxia exposure,and to explore the possible role of interleukin-18 in the pathogenesis of chronic mountain sickness.METHODS:Sixteen healthy male SD rats were randomly divided into two groups:the experimental group was kept in a hypobaric oxygen chamber at a simulated altitude of 5000 m for 28 days,and the control group was kept in a laboratory at an altitude of 2260 m for 28 days.The blood routine tests of the two groups of rats were performed.The proportion of CD71^(+)nucleated erythrocytes in the bone marrow of the two groups of rats was determined by flow cytometry.The expressions of interleukin 18 mRNA and protein in CD71^(+)nucleated erythrocytes in the bone marrow of the two groups of rats were determined by RT-qPCR and western blot assay.The expressions of interleukin 18 protein in the sternum of the two groups of rats were determined by immunofluorescence.The levels of interleukin 18 in the peripheral blood and bone marrow supernatant of the two groups of rats were determined by ELISA.RESULTS AND CONCLUSION:(1)The indexes of erythrocyte count,hemoglobin,hematocrit,and mean hemoglobin content in peripheral blood of the experimental group were higher than those of the control group(P<0.05).(2)The proportion in bone marrow CD71^(+)erythroblasts was significantly higher in the experimental group than that in the control group(P<0.05).(3)RT-qPCR results showed that the expression of interleukin 18 mRNA in CD71^(+)nucleated eryth

关 键 词：白细胞介素18 低氧 骨髓 有核红细胞 血红蛋白 外周血 红细胞 工程化细胞 

分 类 号：R459.9[医药卫生—治疗学] R331[医药卫生—临床医学] R599