不同胃液环境下纳米塑料的凝聚动力学  

Aggregation Kinetics of Nanoplastics in Different Gastric Fluid Environments

作　　者：王星艳 罗诗睫 陈澄宇 WANG Xingyan;LUO Shijie;CHEN Chengyu(Guangdong Key Laboratory of Agricultural and Rural Pollution Control and Environmental Safety,College of Resources and Environment,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]华南农业大学资源环境学院广东省农业农村污染治理与环境安全重点实验室,广东广州510642

出　　处：《广东轻工职业技术大学学报》2024年第6期12-19,共8页Journal of Guangdong Industry Polytechnic university

基　　金：广东省珠江人才计划引进创新创业团队(2019ZT08N291);国家自然科学基金(42377418)。

摘　　要：由于纳米塑料(NPs)的广泛分布,其通过口服摄入到胃环境中几乎不可避免。然而胃环境对NPs的凝聚动力学的影响尚不清楚。探讨了不同pH的胃液条件(空腹、进食)及胃液有机、无机组分对NPs凝聚动力学的影响。结果表明,NPs在四种模拟胃液(SGF)的凝聚速率顺序是SGF2>SGF1>SGF4>SGF3。对于不同pH环境,NPs在pH 7的进食条件下的稳定性强于pH 2的空腹环境。三种电解质均会促进NPs的凝聚,且浓度越高,凝聚速率越大。临界聚沉浓度(CCC)的排序为,CaCl2>KCl>NaCl,NaCl对NPs胶体稳定性的影响最大。单独胃蛋白酶的存在对NPs的胶体稳定性几乎没有影响,而NaCl的加入激活了胃蛋白酶导致NPs的强烈凝聚。胃液中胃蛋白酶和NaCl的复合影响显著降低NPs静电斥力,对NPs胶体稳定性影响最大。这些结果为NPs在胃中的迁移归趋和人体摄入的潜在风险提供理论依据。Due to the widespread presence of nanoplastics(NPs),their oral ingestion into the gastric environment is nearly inevitable.However,the impact of the gastric environment on the aggregation kinetics of NPs remains poorly understood.This study investigated the effects of varying pH conditions in gastric fluid(fasting and feeding)and the organic and inorganic components of gastric fluid on the aggregation kinetics of NPs.The results demonstrated that the aggregation rate of NPs in four simulated gastric fluids(SGF)followed the order:SGF2>SGF1>SGF4>SGF3.Under different pH conditions,NPs exhibited greater stability underfeeding conditions at pH 7 compared to the fasting environment at pH 2.All three electrolytes promoted NP aggregation,with higher concentrations resulting in faster aggregation rates.The critical coagulation concentration(CCC)ranked CaCl2>KCl>NaCl,with NaCl having the most significant impact on the colloidal stability of NPs.The presence of pepsin alone had a minimal effect on NP stability.However,the addition of NaCl activated pepsin,leading to substantial aggregation of NPs.The combined effect of pepsin and NaCl in gastric fluid significantly reduced the electrostatic repulsion between NPs,exerting the most pronounced effect on NP colloidal stability.These findings provide a theoretical foundation for understanding the migration of NPs in the stomach and the potential risks associated with human ingestion.

关 键 词：纳米塑料 胶体稳定性 胃液组分 胃蛋白酶 电解质 

分 类 号：X505[环境科学与工程—环境工程]