不同标记二抗在免疫组化检测CD3和CD21的比较研究  

Comparative Study of Different Labeled Secondary Antibodies in Immunohistochemical Detection of CD3 and CD21

作　　者：唐金宝 李佳露 樊含笑 孙启航 段少仪 胡湘翎 苏建明[1] 雷红宇[1] TANG Jinbao;LI Jialu;FAN Hanxiao;SUN Qihang;DUAN Shaoyi;HU Xiangling;SU Jianming;LEI Hongyu(Hu'nan Agricultural University,Changsha Hu'nan 410128,China)

机构地区：[1]湖南农业大学,湖南长沙410128

出　　处：《现代畜牧科技》2024年第12期23-25,共3页Modern Animal Husbandry Science & Technology

基　　金：湖南省自然科学基金项目(2022JJ30022,2022JJ30293);湖南省技术攻关“揭榜挂帅”项目(2021NK1030)。

摘　　要：免疫组化技术在动物疾病诊断和治疗中有重要的作用。试验使用仔猪肠系膜淋巴结和小鼠脾脏石蜡切片,选择3种不同标记物的二抗,即HRP、Polymer-HRP和AIexa FIuor^(TM)488标记物,观察CD3和CD21蛋白的表达情况,并分析结果中CD3和CD21蛋白的平均光密度值。结果显示,3种不同标记二抗的免疫组化方法染色后,都能在淋巴结和脾脏中显示CD3和CD21阳性信号,在酶标二抗中,Polymer-HRP组的平均光密度值比HRP组显著增高,且使用AIexa FIuor^(TM)488标记的二抗也能获得较高的阳性信号,这表明免疫组化检测中使用不同标记的二抗对组织抗原的检测结果有影响。Immunohistochemistry technology plays an important role in the diagnosis and treatment of animal diseases.In this experiment,paraffin-embedded sections of porcine mesenteric lymph nodes and mouse spleen were used to investigate the expression of CD3 and CD21 proteins using three different secondary antibodies(HRP,Polymer HRP and AIexa FIuor^(TM) 488).The results showed that after immunohistochemical staining with three different labeled secondary antibodies,CD3 and CD21 positive signals could be displayed in lymph nodes and spleen.In the experimental groups using enzymes to label secondary antibodies,the average optical density value of the Polymer HRP group was significantly higher than that of the HRP group.And using AIexa FIuor^(TM) 488 to label the secondary antibody in the experimental can also obtain higher positive signals.This indicates that the use of different labeled secondary antibodies in immunohistochemical detection has a certain impact on the detection results of tissue antigens.

关 键 词：免疫 第二抗体 免疫酶法 免疫荧光法 

分 类 号：R446.6[医药卫生—诊断学]