非天然氧化还原辅酶赋能生物制造  

Non-natural redox cofactors empowered biomanufacturing

作　　者：王雪颖[1] 周雍进[1] 赵宗保[1,2] WANG Xueying;ZHOU Yongjin;ZHAO Zongbao(Division of Biotechnology,Dalian Institute of Chemical Physics,CAS,Dalian 116023,Liaoning,China;School of Bioengineering,Dalian University of Technology,Dalian 116024,Liaoning,China)

机构地区：[1]中国科学院大连化学物理研究所生物技术研究部,辽宁大连116023 [2]大连理工大学生物工程学院,辽宁大连116024

出　　处：《化工学报》2024年第11期4037-4047,共11页CIESC Journal

基　　金：国家重点研发计划项目(2019YFA0904900);国家自然科学基金面上项目(22377125);中国科学院关键核心技术攻坚先导专项(XDC0120102)。

摘　　要：生物制造以生物体机能进行大规模化学品加工与合成。这种生产方式相较于传统的化学制造,具有更低的能耗、更少的废物排放和更高的产品附加值,是一种绿色、可持续的生产方式。构建微生物细胞工厂是生物制造的具体实现形式之一。然而,微生物细胞工厂常因胞内辅酶层次的串扰而未能呈现出预期功能。简要介绍调控胞内氧化还原辅酶水平的常规方法及其局限,说明创制非天然辅酶介导系统的必要性。此外,重点以烟酰胺胞嘧啶二核苷酸和烟酰胺单核苷酸为例,介绍如何研制新型生物学元件、氧化还原模块进而设计新的微生物细胞工厂。最后,预测了基于非天然辅酶的生物制造未来发展方向,并分析了所面临的挑战。Biomanufacturing uses the functions of organisms to process and synthesize chemicals on a large scale.Compared to traditional approaches,biomanufacturing method is considered as a green and sustainable one because it normally consumes less energy,generates less wastes yet outputs higher added-value products.It is routine to design and construct microbial cell factories for biomanufacturing applications.However,microbial cell factories often fail to exhibit expected functions due to intrinsic crosstalk mediated by cofactors.This article briefly introduces conventional methods for regulating intracellular redox coenzyme levels and their limitations,highlights the necessity of creating non-natural coenzyme-mediated systems.Furthermore,with nicotinamide cytosine dinucleotide(NCD)and nicotinamide mononucleotide(NMN)as examples,we will discuss how to explore new biological components and redox modules to design new microbial cell factories.Finally,we predict the future directions and analysis the challenges of biomanufacturing based on non-natural cofactors.

关 键 词：生物催化 酶 合成生物学 辅酶工程 非天然氧化还原辅酶 

分 类 号：Q819[生物学—生物工程]