应用于先进生物成像的荧光染料进展  

Advancements of fluorescent dyes for advanced biological imaging applications

作　　者：许宁 乔庆龙 徐兆超[1] XU Ning;QIAO Qinglong;XU Zhaochao(Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,Liaoning,China)

机构地区：[1]中国科学院大连化学物理研究所,辽宁大连116023

出　　处：《化工学报》2024年第11期4082-4094,共13页CIESC Journal

基　　金：国家自然科学基金项目(22225806,22078314)。

摘　　要：生物荧光标记及成像技术的发展,密切依赖于荧光染料的亮度、光稳定性以及生物相容性等关键发光和化学特性,发展新型荧光团和提升发光性能也将推动生物荧光技术的进步。荧光染料的新颖发光特性又紧密关联于它们的分子结构,而染料结构创新的主要策略包括发现全新的荧光团和对传统荧光团母体进行结构改造。本综述从荧光染料的发光-构效关系切入,详细概述了一系列通过分子结构创新而在发光性能上获得显著提升的荧光染料,并讨论了这些染料在先进生物荧光成像等前沿应用中展现出的卓越表现。最后讨论了生物成像领域中荧光染料的发展所面临的挑战。The advancement of fluorescence labeling and imaging techniques is closely dependent on key emission and chemical properties of fluorescent dyes,such as brightness,photostability,and biocompatibility.The development of new fluorescent groups and the enhancement of fluorescence performance are also expected to propel the progress of fluorescence technology.The novel fluorescent properties of dyes are intricately linked to their molecular structure,with the main strategies for structural innovation including the discovery of entirely new fluorescent groups and the structural modification of traditional fluorescent group frameworks.This review delves into the fluorescent structure-activity relationship of fluorescent dyes,detailing a series of fluorescent dyes that have achieved significant improvements in luminous performance through molecular structural innovation.It discusses the outstanding performance exhibited by these dyes in cutting-edge applications,such as advanced bioluminescence imaging.The article concludes with a discussion of the challenges faced by the development of fluorescent dyes in the field of biological imaging.

关 键 词：荧光染料 结构改造 荧光探针 超分辨成像 

分 类 号：O657.3[理学—分析化学]